构建一种有助于在大肠杆菌菌株之间转移recA突变的高频重组（Hfr）菌株。
Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains.
作者信息
Csonka L N, Clark A J
出版信息
J Bacteriol. 1980 Jul;143(1):529-30. doi: 10.1128/jb.143.1.529-530.1980.
Abstract
Strain JC10240 (Hfr PO45 srlC300::Tn10 recA56 thr-300 ilv-318 rpsE300) was constructed. On account of the close linkage of Tn10 to recA56, the latter can be moved to other Escherichia coli (and closely related) strains in transductional or conjugational crosses selecting resistance to tetracycline.
摘要
构建了菌株JC10240（Hfr PO45 srlC300::Tn10 recA56 thr-300 ilv-318 rpsE300）。由于Tn10与recA56紧密连锁，在选择对四环素耐药性的转导或接合杂交中，后者可转移到其他大肠杆菌（及密切相关的）菌株中。
相似文献
1
Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains.构建一种有助于在大肠杆菌菌株之间转移recA突变的高频重组（Hfr）菌株。
J Bacteriol. 1980 Jul;143(1):529-30. doi: 10.1128/jb.143.1.529-530.1980.
2
Convenient construction of recA deletion derivatives of Escherichia coli.大肠杆菌recA缺失衍生物的便捷构建。
J Bacteriol. 1983 Oct;156(1):458-9. doi: 10.1128/jb.156.1.458-459.1983.
3
Mapping nonselectable genes of Escherichia coli by using transposon Tn10: location of a gene affecting pyruvate oxidase.利用转座子Tn10对大肠杆菌的非选择基因进行定位：一个影响丙酮酸氧化酶的基因的定位
J Bacteriol. 1982 Sep;151(3):1279-89. doi: 10.1128/jb.151.3.1279-1289.1982.
4
Transfer of the delta (argF-lac)U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion.通过选择紧密连锁的Tn10插入片段，在大肠杆菌菌株间转移δ（argF-lac）U169突变。
Mol Gen Genet. 1983;192(1-2):293-4. doi: 10.1007/BF00327683.
5
6
Tetracycline-resistance in Escherichia coli; a genetic contribution.大肠杆菌中的四环素抗性：一种遗传作用。
Antonie Van Leeuwenhoek. 1973;39(1):11-20. doi: 10.1007/BF02578837.
7
Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet lght-induced mutations.大肠杆菌K-12中影响辐射敏感性和紫外线诱导突变频率的显性突变（lex）
J Bacteriol. 1972 Nov;112(2):886-93. doi: 10.1128/jb.112.2.886-893.1972.
8
Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.大肠杆菌srl recA区域的物理图谱：Tn10产生的插入和缺失分析
Mol Gen Genet. 1981;183(3):497-504. doi: 10.1007/BF00268771.
9
Modified map positions for lac and the pro markers in Escherichia coli K-12.大肠杆菌K-12中乳糖操纵子（lac）和脯氨酸（pro）标记的修正图谱位置。
J Bacteriol. 1974 Feb;117(2):741-6. doi: 10.1128/jb.117.2.741-746.1974.
10
Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids.通过整合 pSC101 衍生的接合质粒构建的新型大肠杆菌 Hfr 菌株的特性。
J Bacteriol. 1990 Mar;172(3):1436-40. doi: 10.1128/jb.172.3.1436-1440.1990.
引用本文的文献
1
A Single DNA Binding Site of DprA Dimer Is Required to Facilitate RecA Filament Nucleation.DprA二聚体的单个DNA结合位点是促进RecA丝状体成核所必需的。
Int J Mol Sci. 2025 Aug 15;26(16):7873. doi: 10.3390/ijms26167873.
2
RsmG methylation of 16S rRNA affects the function of ribosomal protein uS12.16S核糖体RNA的RsmG甲基化影响核糖体蛋白uS12的功能。
Arch Microbiol. 2025 May 16;207(7):146. doi: 10.1007/s00203-025-04349-5.
3
Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase.人P32T突变型ITP酶的体外和体内定量表征
Biochim Biophys Acta. 2010 Feb;1802(2):269-74. doi: 10.1016/j.bbadis.2009.11.002. Epub 2009 Nov 13.
4
Superoxide protects Escherichia coli from bleomycin mediated lethality.超氧化物可保护大肠杆菌免受博来霉素介导的致死作用。
J Inorg Biochem. 2009 Sep;103(9):1273-7. doi: 10.1016/j.jinorgbio.2009.07.009. Epub 2009 Jul 17.
5
Repair system for noncanonical purines in Escherichia coli.大肠杆菌中非标准嘌呤的修复系统。
J Bacteriol. 2003 May;185(10):3101-10. doi: 10.1128/JB.185.10.3101-3110.2003.
6
Conjugational hyperrecombination achieved by derepressing the LexA regulon, altering the properties of RecA protein and inactivating mismatch repair in Escherichia coli K-12.通过解除LexA调控子的抑制、改变RecA蛋白的特性以及使大肠杆菌K-12中的错配修复失活来实现接合超重组。
Genetics. 2003 Apr;163(4):1243-54. doi: 10.1093/genetics/163.4.1243.
7
Disruption of lolCDE, encoding an ATP-binding cassette transporter, is lethal for Escherichia coli and prevents release of lipoproteins from the inner membrane.编码一种ATP结合盒转运蛋白的lolCDE的破坏对大肠杆菌是致命的，并阻止脂蛋白从内膜释放。
J Bacteriol. 2002 Mar;184(5):1417-22. doi: 10.1128/JB.184.5.1417-1422.2002.
8
Deletion of lolB, encoding an outer membrane lipoprotein, is lethal for Escherichia coli and causes accumulation of lipoprotein localization intermediates in the periplasm.编码外膜脂蛋白的lolB基因缺失对大肠杆菌是致死性的，并导致脂蛋白定位中间体在周质中积累。
J Bacteriol. 2001 Nov;183(22):6538-42. doi: 10.1128/JB.183.22.6538-6542.2001.
9
Identification of eukaryotic peptide deformylases reveals universality of N-terminal protein processing mechanisms.真核生物肽脱甲酰基酶的鉴定揭示了N端蛋白质加工机制的普遍性。
EMBO J. 2000 Nov 1;19(21):5916-29. doi: 10.1093/emboj/19.21.5916.
10
The modality of enterobacterial common antigen polysaccharide chain lengths is regulated by o349 of the wec gene cluster of Escherichia coli K-12.肠杆菌共同抗原多糖链长度的模式由大肠杆菌K-12的wec基因簇的o349调控。
J Bacteriol. 1999 Oct;181(20):6564-8. doi: 10.1128/JB.181.20.6564-6568.1999.
本文引用的文献
1
Escherichia coli K-12 F-prime factors, old and new.大肠杆菌K-12 F-prime因子，新旧情况
Bacteriol Rev. 1972 Dec;36(4):587-607. doi: 10.1128/br.36.4.587-607.1972.
2
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972.
3
Reversal of the mannitol-sorbitol diauxie in Escherichia coli.大肠杆菌中甘露醇 - 山梨醇二次生长现象的逆转
J Bacteriol. 1972 Nov;112(2):840-8. doi: 10.1128/jb.112.2.840-848.1972.
4
Recalibrated linkage map of Escherichia coli K-12.大肠杆菌K-12的重新校准连锁图谱。
Bacteriol Rev. 1976 Mar;40(1):116-67. doi: 10.1128/br.40.1.116-167.1976.
5
Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli K-12 chromosome.转座子Tn10在大肠杆菌K-12染色体的srl recA区域产生的缺失。
Genetics. 1979 Oct;93(2):321-43. doi: 10.1093/genetics/93.2.321.
6
Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.大肠杆菌和噬菌体λ中可转移四环素抗性元件Tn10的特性
Genetics. 1978 Nov;90(3):427-61. doi: 10.1093/genetics/90.3.427.
7
Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae.肺炎克雷伯菌中固氮基因（nif）的精细结构图谱绘制与互补分析
J Bacteriol. 1978 Oct;136(1):253-66. doi: 10.1128/jb.136.1.253-266.1978.
Zotero 插件