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1
Novel system for recognizing and eliminating foreign DNA in Pseudomonas putida.恶臭假单胞菌中识别和消除外源DNA的新系统。
J Bacteriol. 1984 Aug;159(2):678-82. doi: 10.1128/jb.159.2.678-682.1984.
2
Broad-host-range IncP-4 plasmid R1162: effects of deletions and insertions on plasmid maintenance and host range.广宿主范围IncP-4质粒R1162：缺失和插入对质粒维持及宿主范围的影响
J Bacteriol. 1982 Oct;152(1):140-50. doi: 10.1128/jb.152.1.140-150.1982.
3
The use of plasmid R1162 and derivatives for gene cloning in the methanol-utilizing Pseudomonas AM1.质粒R1162及其衍生物在利用甲醇的假单胞菌AM1中用于基因克隆。
Mol Gen Genet. 1980;178(2):375-80. doi: 10.1007/BF00270487.
4
[Cloning and localization of the replication and mobilization regions in the D-plasmid of Pseudomonas putida pBS286 (IncP-9) with a broad host range].[恶臭假单胞菌pBS286（IncP-9）具有广泛宿主范围的D质粒中复制和转移区域的克隆与定位]
Genetika. 1988 Jun;24(6):980-92.
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Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli.食油假单胞菌烷烃利用系统在恶臭假单胞菌和大肠杆菌中的可控功能性表达。
J Biol Chem. 1987 Dec 25;262(36):17712-8.
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Molecular cloning of the Pseudomonas putida glyoxalase I gene in Escherichia coli.恶臭假单胞菌乙二醛酶I基因在大肠杆菌中的分子克隆
Biochem Biophys Res Commun. 1987 Sep 15;147(2):831-8. doi: 10.1016/0006-291x(87)91005-9.
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Pseudomonas chromosomal replication origins: a bacterial class distinct from Escherichia coli-type origins.铜绿假单胞菌染色体复制起点：一类不同于大肠杆菌型起点的细菌类型。
Proc Natl Acad Sci U S A. 1990 Feb;87(4):1278-82. doi: 10.1073/pnas.87.4.1278.
8
Analysis of the vegetative replication origin of broad-host-range plasmid RK2 by transposon mutagenesis.通过转座子诱变分析广宿主范围质粒RK2的营养复制起点
Plasmid. 1986 Mar;15(2):132-46. doi: 10.1016/0147-619x(86)90049-1.
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In vivo formation of gene fusions in Pseudomonas putida and construction of versatile broad-host-range vectors for direct subcloning of Mu d1 and Mu d2 fusions.恶臭假单胞菌中基因融合的体内形成以及用于直接亚克隆Mu d1和Mu d2融合体的通用广宿主载体的构建。
Appl Environ Microbiol. 1987 Jul;53(7):1649-54. doi: 10.1128/aem.53.7.1649-1654.1987.
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[Transformation of Escherichia coli and different species of Pseudomonas by a plasmid DNA isolated from Pseudomonas testosteroni].[从睾丸酮假单胞菌分离的质粒DNA对大肠杆菌和不同种假单胞菌的转化]
C R Seances Acad Sci III. 1983;297(1):25-8.

引用本文的文献

1
Recombinant plasmid conferring proline overproduction and osmotic tolerance.赋予脯氨酸过量生产和渗透耐受性的重组质粒。
Appl Environ Microbiol. 1985 Aug;50(2):441-6. doi: 10.1128/aem.50.2.441-446.1985.
2
Integrative suppression of dnaA46 by broad host-range plasmid R1162.广宿主范围质粒R1162对dnaA46的整合抑制作用。
Mol Gen Genet. 1988 Dec;215(1):139-45. doi: 10.1007/BF00331315.

本文引用的文献

1
Broad-host-range IncP-4 plasmid R1162: effects of deletions and insertions on plasmid maintenance and host range.广宿主范围IncP-4质粒R1162：缺失和插入对质粒维持及宿主范围的影响
J Bacteriol. 1982 Oct;152(1):140-50. doi: 10.1128/jb.152.1.140-150.1982.
2
Genetic organization of the broad-host-range IncP-1 plasmid R751.广宿主范围IncP-1质粒R751的基因组织。
J Bacteriol. 1980 Sep;143(3):1362-73. doi: 10.1128/jb.143.3.1362-1373.1980.
3
A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder.一种使用碱提取法并结合玻璃粉进行大规模分离高纯度质粒DNA的方法。
Anal Biochem. 1982 Apr;121(2):382-7. doi: 10.1016/0003-2697(82)90497-3.
4
Construction and characterization of multicopy plasmids containing the entire F transfer region.包含完整F转移区域的多拷贝质粒的构建与表征
Plasmid. 1980 Nov;4(3):292-304. doi: 10.1016/0147-619x(80)90068-2.
5
Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides.赋予链霉素和磺胺类药物连锁抗性的质粒的脱氧核糖核酸分子量及同源性比较
J Bacteriol. 1974 Nov;120(2):618-30. doi: 10.1128/jb.120.2.618-630.1974.
6
R factor transfer in Rhizobium leguminosarum.根瘤菌中R因子的转移
J Gen Microbiol. 1974 Sep;84(1):188-98. doi: 10.1099/00221287-84-1-188.
7
Transposition of ampicillin resistance from RP4 to other replicons.氨苄青霉素抗性从RP4转座至其他复制子。
Mol Gen Genet. 1974;132(1):31-40. doi: 10.1007/BF00268228.
8
Trimethoprim R factors in enterobacteria from clinical specimens.临床标本中肠杆菌科细菌的甲氧苄啶R因子
J Med Microbiol. 1974 May;7(2):169-77. doi: 10.1099/00222615-7-2-169.
9
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972.
10
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.细菌中的非染色体抗生素抗性：R 因子 DNA 对大肠杆菌的遗传转化
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4. doi: 10.1073/pnas.69.8.2110.

恶臭假单胞菌中识别和消除外源DNA的新系统。

Novel system for recognizing and eliminating foreign DNA in Pseudomonas putida.

作者信息

Kim K, Meyer R J

出版信息

J Bacteriol. 1984 Aug;159(2):678-82. doi: 10.1128/jb.159.2.678-682.1984.

DOI:10.1128/jb.159.2.678-682.1984
PMID:6086582
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215697/
Abstract

Derivatives of pQSR49 (R1162::Tn1) containing cloned fragments of Escherichia coli chromosomal DNA are stable in E. coli but unstable in Pseudomonas putida. Similar derivatives containing P. putida chromosomal DNA are stable in both species. Instability is a consequence of plasmid loss during growth and is not due to death or inhibition of growth of plasmid-containing cells. Average copy numbers per cell of the unstable hybrid plasmids are similar to that of pQSR49, indicating that instability is not the result of reduced replication of plasmid DNA. A plasmid unrelated to pQSR49, RK2, becomes unstable in the presence of the unstable hybrids but only when it also contains foreign DNA. The data suggest an inducible mechanism in P. putida active in the elimination of plasmid-borne foreign DNA from the cell.

摘要

含有大肠杆菌染色体DNA克隆片段的pQSR49（R1162::Tn1）衍生物在大肠杆菌中稳定，但在恶臭假单胞菌中不稳定。含有恶臭假单胞菌染色体DNA的类似衍生物在这两个物种中都稳定。不稳定性是生长过程中质粒丢失的结果，而不是由于含质粒细胞的死亡或生长抑制。不稳定杂交质粒的每个细胞平均拷贝数与pQSR49相似，表明不稳定性不是质粒DNA复制减少的结果。与pQSR49无关的质粒RK2，在存在不稳定杂交体时变得不稳定，但仅当它也含有外源DNA时才会如此。数据表明恶臭假单胞菌中存在一种诱导机制，可有效从细胞中消除质粒携带的外源DNA。