Zhuang Y-P, Zhu Y-P, Wang H-Y, Sun L, Zhang J, Hao Y-P, Wang L
Department of Radiology, Jiangsu Cancer Institute and Hospital, Nanjing, Jiangsu, China.
Nanjing Personal Oncology Biological Technology Co. Ltd., Nanjing, Jiangsu, China.
Braz J Med Biol Res. 2017 May 18;50(6):e6000. doi: 10.1590/1414-431X20176000.
This study aimed to investigate the feasibility of the establishment of a human cancer xenograft model using samples from computed tomography (CT)-guided percutaneous biopsy. Fresh tumor tissues obtained from 10 cancer patients by CT-guided percutaneous biopsy were subcutaneously inoculated into NOD-Prkdcem26Il2rgem26Nju (NCG) mice to establish human patient-derived tumor xenograft (PDTX) models. The formation of first and second generation xenografts was observed, and tumor volume was recorded over time. Tumor tissue consistency between the PDTX model and primary tumors in patients was compared using H&E staining and immunohistochemistry. Pharmacodynamic tests of clinically used chemotherapeutic drugs were conducted on second generation xenografts, and their effects on tumor growth and body weight were observed. CT-guided percutaneous biopsy samples were successfully collected from 10 patients with advanced cancers. The PDTX model was established in mice using tumor samples obtained from 4 cancer patients, including one small cell carcinoma sample, two adenocarcinoma samples, and one squamous cell carcinoma sample. The success rate was 40%. The obtained PDTX model maintained a degree of differentiation, and morphological and structural characteristics were similar to primary tumors. The pharmacodynamic test of chemotherapeutic drugs in the PDTX model revealed a therapeutic effect on tumor growth, as expected. CT-guided percutaneous biopsy samples can be effectively used to establish a PDTX model, and test these chemotherapy regimens.
本研究旨在探讨使用计算机断层扫描（CT）引导下经皮活检样本建立人癌异种移植模型的可行性。通过CT引导下经皮活检从10例癌症患者获取的新鲜肿瘤组织，皮下接种到NOD-Prkdcem26Il2rgem26Nju（NCG）小鼠中，以建立人患者来源的肿瘤异种移植（PDTX）模型。观察第一代和第二代异种移植瘤的形成，并随时间记录肿瘤体积。使用苏木精和伊红（H&E）染色及免疫组织化学比较PDTX模型与患者原发性肿瘤之间的肿瘤组织一致性。对第二代异种移植瘤进行临床使用的化疗药物的药效学试验，并观察其对肿瘤生长和体重的影响。成功从10例晚期癌症患者中采集了CT引导下经皮活检样本。使用从4例癌症患者获得的肿瘤样本在小鼠中建立了PDTX模型，包括1例小细胞癌样本、2例腺癌样本和1例鳞状细胞癌样本。成功率为40%。所获得的PDTX模型保持了一定程度的分化，形态和结构特征与原发性肿瘤相似。PDTX模型中化疗药物的药效学试验显示对肿瘤生长有预期的治疗效果。CT引导下经皮活检样本可有效用于建立PDTX模型并测试这些化疗方案。
