关于建立人源肿瘤异种移植模型的初步研究
A Preliminary Study on the Establishment of the PDTX Model.
作者信息
Liu Yong, Zhu Yan-Ping, Cai Ming-Zhi, Ke Bin, Li Bin, Liu Ning, Xue Qiang, Zhan Hong-Jie, Deng Jing-Yu, Zhang Li, Hao Yan-Peng, Wang Zhi-Qiang, Wang Li, Liang Han
机构信息
Department of Gastroenterology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Centre for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, People's Republic of China.
Nanjing Personal Oncology Biological Technology Co. Ltd., Nanjing 211100, Jiangsu, People's Republic of China.
出版信息
Cancer Manag Res. 2020 Mar 17;12:1969-1979. doi: 10.2147/CMAR.S230668. eCollection 2020.
OBJECTIVE
The current study aims to explore the establishment of the patient-derived tumor xenograft (PDTX) model.
MATERIALS AND METHODS
Twenty patients with gastric cancer, 10 males and 10 females, were enrolled in the current study. Firstly, the volume, invasion and metastasis of the xenografts were observed. Subsequently, the correlation between tumor tissues of the PDTX mouse model and the patients' primary tumor tissues was evaluated by pathological H&E staining and immunohistochemistry.
RESULTS
The results showed that the PDTX models corresponding to 15 of the 20 patients were successfully established, and the success rate of PDTX model establishment was 75%. Furthermore, the PDTX models maintained the differentiation degree, morphological characteristics and structural characteristics of tumor cells.
CONCLUSION
A PDTX model can be used as a substitute for cancer patients in clinical practice and may be suitable for clinical pharmacodynamic screening and new drug development.
目的
本研究旨在探索建立患者来源的肿瘤异种移植（PDTX）模型。
材料与方法
本研究纳入了20例胃癌患者，其中男性10例，女性10例。首先，观察异种移植瘤的体积、侵袭和转移情况。随后，通过病理苏木精-伊红（H&E）染色和免疫组织化学评估PDTX小鼠模型的肿瘤组织与患者原发肿瘤组织之间的相关性。
结果
结果显示，20例患者中有15例成功建立了PDTX模型，PDTX模型建立成功率为75%。此外，PDTX模型保持了肿瘤细胞的分化程度、形态特征和结构特征。
结论
PDTX模型可在临床实践中替代癌症患者，可能适用于临床药效学筛选和新药开发。
Zotero 插件