Evaluation of Herb-Drug Interactions of Fruit Extracts.

BACKGROUND

(Rhamnaceae) fruits are popularly used as herbal medicines or dietary supplements in Asian countries due to functions such as liver protection and detoxification from alcohol poisoning. Accordingly, it is very likely for dietary supplemental products, including fruit extracts, to be taken with prescription drugs.

OBJECTIVE

In this study, possible food-drug interactions involving fruit extracts were evaluated based on the inhibition of cytochrome P450 (CYP) enzyme activity.

MATERIAL AND METHODS

The water extract of fruit extracts was incubated in human liver microsomes with CYP-specific substrates. The formation of the CYP-specific metabolites was measured using liquid chromatography-tandem mass spectrometry.

RESULTS

fruit extracts showed negligible effects on seven CYP isozyme activities at all concentrations tested.

CONCLUSION

This result suggests that fruit extracts may have minimal pharmacokinetic interactions with coadministered drugs through the modulation of CYP enzymes.

SUMMARY

Food-drug interactions involving fruit extracts were evaluated.The inhibition of CYPs by extracts was tested. extracts showed negligible effects on CYP activities. extracts may have minimal pharmacokinetic interactions with co-administered drugs. CYP: cytochrome P450 enzymes, HPLC: High performance liquid chromatography, LC-MS/MS : liquid chromatography-tandem mass spectrometry, MRM: multiple-reaction monitoring.