Kim Hye-Lin, Sim Jung-Eun, Choi Hyun-Myung, Choi In-Young, Jeong Mi-Young, Park Jinbong, Jung Yunu, Youn Dong-Hyun, Cho Jung-Hee, Kim Ji-Hyun, Hwang Min-Woo, Jin Jong-Sik, Hong Seung-Heon, Cho Hyun-Woo, Um Jae-Young
College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, Republic of Korea.
Food Funct. 2014 Nov;5(11):2961-8. doi: 10.1039/c4fo00470a.
Hovenia dulcis Thunb. is well known as a treatment for liver disease. Several studies have demonstrated that extracts of Hovenia dulcis Thunb. or its purified compounds can serve as detoxifying agents for alcohol poisoning. However, its anti-obesity effect has not been reported thus far. In this study, the anti-obesity effect of water extracts from the fruits or stems of Hovenia dulcis Thunb. was examined in 3T3-L1 preadipocytes. The cellular lipid contents in 3T3-L1 adipocytes were assessed by Oil Red O staining. Fruits of Hovenia dulcis Thunb. (FHD) significantly inhibit lipid accumulation during adipogenesis in a dose-dependent manner, but not stems of Hovenia dulcis Thunb. FHD (100 μg ml(-1)) significantly down-regulates the expression of the peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding protein-α, adipocyte fatty acid-binding protein 2, adiponectin, and resistin, and the inhibition rates were 29.33%, 54.36%, 34.5%, 55.69%, and 60.39%, respectively. In addition, FHD (100 μg ml(-1)) also up-regulates the phosphorylation of AMP-activated protein kinase (AMPK)-α, liver kinase B1 as a major AMPK kinase, and the downstream substrate acetyl-CoA carboxylase, and the inhibition rates were 43.52%, 38.25%, and 20.39%, respectively. These results indicate that FHD has a significant anti-obesity effect through the modulation of the AMPK pathway, suggesting that FHD has a potential benefit in preventing obesity.
枳椇子作为治疗肝病的药物而广为人知。多项研究表明，枳椇子提取物或其纯化化合物可作为酒精中毒的解毒剂。然而，其抗肥胖作用迄今尚未见报道。在本研究中，检测了枳椇子果实或茎的水提取物对3T3-L1前脂肪细胞的抗肥胖作用。通过油红O染色评估3T3-L1脂肪细胞中的细胞脂质含量。枳椇子果实（FHD）以剂量依赖性方式显著抑制脂肪生成过程中的脂质积累，但枳椇子茎则无此作用。FHD（100μg/ml）显著下调过氧化物酶体增殖物激活受体γ、CCAAT/增强子结合蛋白α、脂肪细胞脂肪酸结合蛋白2、脂联素和抵抗素的表达，抑制率分别为29.33%、54.36%、34.5%、55.69%和60.39%。此外，FHD（100μg/ml）还上调了AMP激活蛋白激酶（AMPK）α、作为主要AMPK激酶的肝激酶B1及其下游底物乙酰辅酶A羧化酶的磷酸化，抑制率分别为43.52%、38.25%和20.39%。这些结果表明，FHD通过调节AMPK途径具有显著的抗肥胖作用，提示FHD在预防肥胖方面具有潜在益处。
