Silva Aline M R, Ferreira Nayara L O, Oliveira Anselmo E, Borges Leonardo L, Conceição Edemilson C
Faculty of Pharmacy Research, Laboratory of Natural Products, School of Pharmacy, Federal University of Goiás, Goiânia, Goiás, Brazil.
Chemistry Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
Pharmacogn Mag. 2017 Apr-Jun;13(50):270-274. doi: 10.4103/0973-1296.204555. Epub 2017 Apr 18.
belongs to the Asteraceae family. The leaves of have been studied lately because of the presence of tagitinin C.
Looking for an easy and inexpensive method to extract tagitinin C from leaves, this work aims to conduct a screening to evaluate the influence of different experimental factors using the dynamic maceration and ultrasound-assisted extraction methods with 2 factorial design based on response surface methodology in enhancing this chemical marker extraction.
The experimental factors were: extraction time (ET) of 30 and 60 minutes, solid: liquid ratio (SLR) of 5 and 10 grams/grams and ethanolic strength (ES) 48 and 96% (w/w). The experiments were done tripled. The content of tagitinin C in each produced extract was quantified by HPLC method.
The highest concentrations of tagitinin C obtained under the experimental design were 0.53 mg/mL and 0.71 mg/mL, respectively for dynamic maceration (DM) and ultrasound-assisted extraction (UAE) from powdered leaves. For the UAE method, the main parameter for higher contents of tagitinin C was the solid: liquid ratio, followed by the ethanolic strength, and the extraction time was not significant for this method. As for the DM method, all the parameters (SLR, ES, and ET) were significant for a higher content of tagitinin C.
Based on the obtained results, it was revealed that the ultrasound-assisted extraction was more effective than dynamic maceration for tagitinin C extraction from powdered leaves.
leaves possess tagitinin C, a sesquiterpene lactone, as an important secondary metabolite with several biological activities, such as antimalarial, gastroprotective, chemotherapeutic adjuvants, and toxic activities.Ultrasound-assisted extraction was more effective to obtain higher levels of tagitinin C when compared with dynamic maceration extraction.Factorial design can be employed as a screening tool to find the effects of factors investigated in the extraction processes. DME: dynamic maceration extraction, UAE: ultrasound-assisted extraction, ET: extraction time, ES: ethanolic strength, SLR: solid:liquid ratio, Tag C: tagitinin C, HPLC: high-performance liquid chromatography.
属于菊科。由于含有tagitinin C，其叶子最近受到了研究。
为了寻找一种从其叶子中提取tagitinin C的简便且低成本的方法，本研究旨在进行筛选，以评估不同实验因素的影响，采用基于响应面法的二因素设计的动态浸渍法和超声辅助提取法来提高这种化学标志物的提取率。
实验因素包括：提取时间（ET）30分钟和60分钟、固液比（SLR）5克/克和10克/克以及乙醇浓度（ES）48%和96%（w/w）。实验重复三次。通过高效液相色谱法对各提取物中tagitinin C的含量进行定量。
在实验设计下，从其粉末状叶子中进行动态浸渍（DM）和超声辅助提取（UAE）分别获得的tagitinin C最高浓度为0.53毫克/毫升和0.71毫克/毫升。对于超声辅助提取法，获得较高含量tagitinin C的主要参数是固液比，其次是乙醇浓度，提取时间对该方法不显著。至于动态浸渍法，所有参数（SLR、ES和ET）对于获得较高含量的tagitinin C均有显著影响。
根据所得结果表明，超声辅助提取法从其粉末状叶子中提取tagitinin C比动态浸渍法更有效。
其叶子含有tagitinin C，一种倍半萜内酯，作为一种重要的次生代谢产物，具有多种生物活性，如抗疟疾、胃保护、化疗辅助和毒性活性。与动态浸渍提取相比，超声辅助提取能更有效地获得更高水平的tagitinin C。析因设计可作为一种筛选工具，以找出提取过程中所研究因素的影响。DME：动态浸渍提取，UAE：超声辅助提取，ET：提取时间，ES：乙醇浓度，SLR：固液比，Tag C：tagitinin C，HPLC：高效液相色谱法。
