Anti-Differentiation Effect of B, D- limonoids of .

BACKGROUND

Obesity is a pathological state caused by abnormal or excessive accumulation of fat. JACQ., known as West Indian mahogany, is a medium-sized semi-evergreen tree belonging to Meliaceae. Their seeds are used in Indonesian folk medicine as a treatment for hypertension, diabetes, malaria, and it also has anti-feedant activities. The major components of are B, D-seco limonoids, a type of irregular triterpenes are well known.

OBJECTIVE

We tried to find the bioactive components, which have the inhibitory activity on adipocyte differentiation from the seeds of .

MATERIAL AND METHODS

3T3-L1 cells, derived from mouse preadipocyte, are widely used in studying adipogenesis process. In this study, we used 3T3-L1 cells to find natural products with the inhibitory activity on adipocyte differentiation. seeds were dried and extracted with 100% MeOH.

RESULTS

The methanolic extract was fractionated by bioassay-guided method to give nine B, D-seco limonoids (1-9) with slight structural modifications. Among nine compounds, compounds 4, 6 and 8 exhibited significant inhibitory effects of cell differentiation on 3T3-L1 cells. Those compounds have tigloyl residue at C-3 in common. Besides, compounds with no tigloyl residue at C-3 showed insignificant effect. Nevertheless, not all compounds with tigloyl residue at C-3 exerted significant inhibitory effect.

CONCLUSION

These results suggested that tigloyl residue at C-3 may play a role in the anti-proliferative activity on a dipogenesis and the refined extract of may have a potential to be developed as a therapeutic agent to treat obesity.

SUMMARY

Nine irregular seco-limonoids were isolated from .Total extract and CHCl3 fraction of showed the significant inhibitory activities on 3T3-L1 cell differentiation.A tigloyl residue at C-3 in an aglycone may play a role in the anti-proliferative activity on adipogenesis.