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来自商业盐的极端嗜盐古菌和细菌群落的多样性

Diversity of Extremely Halophilic Archaeal and Bacterial Communities from Commercial Salts.

作者信息

Gibtan Ashagrie, Park Kyounghee, Woo Mingyeong, Shin Jung-Kue, Lee Dong-Woo, Sohn Jae Hak, Song Minjung, Roh Seong Woon, Lee Sang-Jae, Lee Han-Seung

机构信息

Major in Food Biotechnology, Division of Bioindustry, Silla UniversityBusan, South Korea.

Department of Korean Cusine, Jeonju UniversityJeonju, South Korea.

出版信息

Front Microbiol. 2017 May 10;8:799. doi: 10.3389/fmicb.2017.00799. eCollection 2017.

Abstract

Salting is one of the oldest food preservation techniques. However, salt is also the source of living halophilic microorganisms that may affect human health. In order to determine the microbial communities of commercial salts, an investigation were done using amplicon sequencing approach in four commercial salts: Ethiopian Afdera salt (EAS), Ethiopian rock salt (ERS), Korean Jangpan salt (KJS), and Korean Topan salt (KTS). Using domain-specific primers, a region of the 16S rRNA gene was amplified and sequenced using a Roche 454 instrument. The results indicated that these microbial communities contained 48.22-61.4% Bacteria, 37.72-51.26% Archaea, 0.51-0.86% Eukarya, and 0.005-0.009% unclassified reads. Among bacteria, the communities in these salts were dominated by the phyla , and . Of the archaea, 91.58% belonged to the class , whereas the remaining 7.58, 0.83, and 0.01% were , and , respectively. This comparison of microbial diversity in salts from two countries showed the presence of many archaeal and bacterial genera that occurred in salt samples from one country but not the other. The bacterial genera and were found only in Korean and Ethiopian salts, respectively. This study indicated the occurrence and diversity of halophilic bacteria and archaea in commercial salts that could be important in the gastrointestinal tract after ingestion.

摘要

盐腌是最古老的食品保存技术之一。然而,盐也是可能影响人类健康的嗜盐微生物的来源。为了确定市售盐中的微生物群落,采用扩增子测序方法对四种市售盐进行了调查:埃塞俄比亚阿夫代拉盐(EAS)、埃塞俄比亚岩盐(ERS)、韩国酱盘盐(KJS)和韩国托潘盐(KTS)。使用针对特定结构域的引物,扩增16S rRNA基因的一个区域,并使用罗氏454仪器进行测序。结果表明,这些微生物群落包含48.22% - 61.4%的细菌、37.72% - 51.26%的古菌、0.51% - 0.86%的真核生物以及0.005% - 0.009%的未分类读数。在细菌中,这些盐中的群落以门 、 和 为主。在古菌中,91.58%属于 纲,而其余的7.58%、0.83%和0.01%分别属于 、 和 。对来自两个国家的盐中微生物多样性的比较表明,存在许多在一个国家的盐样品中出现而在另一个国家未出现的古菌和细菌属。细菌属 和 分别仅在韩国盐和埃塞俄比亚盐中发现。这项研究表明了市售盐中嗜盐细菌和古菌的存在及多样性,这些在摄入后可能对胃肠道很重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a07/5423978/ef38ba715852/fmicb-08-00799-g0001.jpg

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