Li Lu, Hao Chunbo, Wang Lihua, Pei Lixin
Wei Sheng Wu Xue Bao. 2015 Apr 4;55(4):412-24.
We characterized procaryotic biodiversity, community structure and the relationship between the community structure and environmental factors of salt lakes in Badain Jaran desert, Inner Mongolia, China.
We constructed 16S rRNA gene clone libraries by molecular biology techniques to analyze the procaryotic phylogenetic relationships, and used R language to compare the community structure of haloalkalophiles in the salt lakes.
Water in this region has a high salinity ranging from 165 to 397 g/L. The water is strongly alkaline with pH value above 10. The microbial diversity and community structure of the salt lakes are obviously different. The diversity of bacteria is more abundant than that of archaea. The main categories of bacteria in the samples are Gammaproteobacteria, Bacteroidetes, Alphaproteobacteria, Firmicute and Verrucomicrobia, whereas all archaea only belong to Halobacteriaceae of Euryarchaeota.
Salinity is the most important environmental factor influencing the bacterial community structure, whereas the archaea community structure was influenced comprehensively by multiple environmental factors.
我们对中国内蒙古巴丹吉林沙漠盐湖的原核生物多样性、群落结构以及群落结构与环境因素之间的关系进行了表征。
我们通过分子生物学技术构建16S rRNA基因克隆文库，以分析原核生物的系统发育关系，并使用R语言比较盐湖中嗜盐碱菌的群落结构。
该地区水体盐度较高，范围为165至397克/升。水体呈强碱性，pH值高于10。盐湖的微生物多样性和群落结构明显不同。细菌的多样性比古菌更丰富。样品中细菌的主要类别为γ-变形菌纲、拟杆菌门、α-变形菌纲、厚壁菌门和疣微菌门，而所有古菌仅属于广古菌门的嗜盐菌科。
盐度是影响细菌群落结构的最重要环境因素，而古菌群落结构受到多种环境因素的综合影响。
