a School of Biomedical Engineering , Shanghai Jiao Tong University , Shanghai , People's Republic of China.
b Shanghai Key Laboratory of Psychotic Disorders , Shanghai Mental Health Center , Shanghai , People's Republic of China.
Int J Hyperthermia. 2018 May;34(3):261-272. doi: 10.1080/02656736.2017.1332394. Epub 2017 Jun 6.
In our previous studies, a novel tumour therapeutic modality of the cryo-thermal therapy has been developed leading to long-term survival in 4T1 murine mammary carcinoma model. The cryo-thermal therapy induced the strong acute inflammatory response and IL-6 was identified in an acute profile. In this study, we found that the cryo-thermal therapy triggered robust acute inflammatory response with high expression of IL-6 locally and systemically. The phenotypic maturation of dendritic cells (DCs) was induced by acute IL-6 following the treatment. The mature DCs promoted CD4 T cell differentiation. Moreover, the production of interferon γ (IFN γ) in the serum and CD4 T cells were both abrogated by IL-6 neutralisation following the treatment. Our findings revealed that the cryo-thermal therapy-induced acute IL-6 played an important role in initiating the cascading innate and adaptive anti-tumour immune responses, resulting in CD4 T cell differentiation. It would be interesting to investigate acute IL-6 as an early indicator in predicating tumour therapeutic effect.
在我们之前的研究中，开发了一种新型的肿瘤治疗方式——冷冻-热疗，该方法能使 4T1 鼠乳腺癌模型长期存活。冷冻-热疗会引起强烈的急性炎症反应，并且 IL-6 会呈现出急性特征。在这项研究中，我们发现冷冻-热疗会引发强烈的急性炎症反应，导致局部和全身的 IL-6 表达水平升高。急性 IL-6 会诱导树突状细胞（DC）的表型成熟。成熟的 DC 会促进 CD4 T 细胞的分化。此外，治疗后通过中和 IL-6，血清和 CD4 T 细胞中的干扰素 γ（IFNγ）的产生都被阻断。我们的研究结果表明，冷冻-热疗诱导的急性 IL-6 在启动级联的固有和适应性抗肿瘤免疫反应中发挥了重要作用，从而促进 CD4 T 细胞的分化。研究急性 IL-6 作为预测肿瘤治疗效果的早期指标将是一件很有趣的事。
