Vayne Caroline, Deroche Luc, Drillaud Nicolas, Patin Franck, Bruno Clément, Maillot François, Labarthe François, Corcia Philippe, Benz-de Bretagne Isabelle, Andres Christian R, Emond Patrick, Blasco Hélène
Laboratoire de biochimie et biologie moléculaire, CHRU Tours, France.
Laboratoire de biochimie et biologie moléculaire, CHRU Tours, France, Inserm U930, Université François Rabelais, Tours, France.
Ann Biol Clin (Paris). 2017 Jun 1;75(3):334-338. doi: 10.1684/abc.2017.1248.
Homocysteine (Hcy) is monitored in a growing number of diseases and requires a rapid and reliable method to measure its concentration in routine practice. We validated a new mass spectrometry method to measure plasma Hcy concentration and to determinate our own targeted concentrations according to COFRAC (French accreditation committee) recommendations. We collected the Hcy concentrations measured in the laboratory from 2014 to 2015 and we compared the values between different clinical groups. We obtained excellent performances of reproducibility, sensitivity (coefficient of variation <10%). We determined new ranges of normal values 12.4 [11.8-13.0] μmol/L. This new method is thus rapid, robust and suitable for its use in routine practice to respond to the increase of prescriptions.
越来越多的疾病需要监测同型半胱氨酸（Hcy），这就需要一种快速可靠的方法来在常规实践中测量其浓度。我们验证了一种新的质谱方法，用于测量血浆Hcy浓度，并根据法国认可委员会（COFRAC）的建议确定我们自己的目标浓度。我们收集了2014年至2015年实验室测量的Hcy浓度，并比较了不同临床组之间的值。我们获得了出色的重现性和灵敏度（变异系数<10%）。我们确定了新的正常值范围为12.4 [11.8 - 13.0] μmol/L。因此，这种新方法快速、稳健，适用于常规实践，以应对处方量的增加。
