筛选对肠杆菌、假单胞菌属和葡萄球菌属具有抗菌活性的植物

Screening of plants with antimicrobial activity against enterobacteria, Pseudomonas spp. and Staphylococcus spp.

作者信息

Girondi Camila Miorelli, de Oliveira Analu Barros, Prado Jackeline Alves, Koga-Ito Cristiane Yumi, Borges Aline Chiodi, Botazzo Delbem Alberto Carlos, Alves Pereira Daniel Freitas, Salvador Marcos José, Brighenti Fernanda Lourenção

机构信息

Department of Environmental Engineering & Oral Biopathology Graduate Program, Universidade Estadual Paulista (UNESP), São José dos Campos Institute of Science & Technology, Avenida Engenheiro Francisco José Longo, 777, São José dos Campos, SP 12245-000, Brazil.

Department of Orthodontics & Pediatric Dentistry, Universidade Estadual Paulista (UNESP), Araraquara Dental School, R Humaitá, 1680, Araraquara, SP 14801-903, Brazil.

出版信息

Future Microbiol. 2017 Jun;12:671-681. doi: 10.2217/fmb-2016-0129. Epub 2017 May 25.

DOI:10.2217/fmb-2016-0129

PMID:28541127

Abstract

AIM

This study screened plants for antibacterial properties against bacteria of medical importance.

MATERIALS & METHODS: 60 extracts were obtained from the leaves of ten plants (Jatropha weddelliana, Attalea phalerata, Buchenavia tomentosa, Croton doctoris, Mouriri elliptica, Mascagnia benthamiana, Senna aculeata, Unonopis guatterioides, Allagoptera leucocalyx and Bactris glaucescens) using different extraction methods: A) Ethanol 70°C/72 h; B) Water/5 min/100°C; C) Water/1 h/55°C; D) Water/72 h; E) Hexane/72 h and F) Ethanol 99°C/72 h. Enterobacteria/Pseudomonas and staphylococci reference strains and 201 clinical isolates were used. Primary screening was done using agar well-diffusion assay. MIC/minimum bactericidal concentration and chemical characterization were determined.

RESULTS

Extracts 5F and 3A showed the best MIC/minimum bactericidal concentration against clinical isolates and showed the presence of phenols.

CONCLUSION

The present study demonstrated that Mouriri elliptica and Buchenavia tomentosa were the most active plants against the studied bacteria.

摘要

目的

本研究筛选对具有医学重要性的细菌具有抗菌特性的植物。

材料与方法

使用不同提取方法从十种植物（韦氏麻风树、具鳞鱼尾葵、绒毛布氏木、多克氏巴豆、椭圆穆氏木、本氏马斯卡尼亚木、尖刺番泻、瓜特吉乌诺皮斯木、白萼三齿棕和青棕）的叶子中获得60种提取物：A）70°C乙醇/72小时；B）水/5分钟/100°C；C）水/1小时/55°C；D）水/72小时；E）己烷/72小时；F）99°C乙醇/72小时。使用肠杆菌/假单胞菌和葡萄球菌参考菌株以及201株临床分离株。采用琼脂孔扩散法进行初步筛选。测定了最低抑菌浓度/最低杀菌浓度和化学特性。