Hasan Md Riasat, Takebe Hiroaki, Shalehin Nazmus, Obara Nobuko, Saito Takashi, Irie Kazuharu
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-gun, Hokkaido, Japan.
Division of Histology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-gun, Hokkaido, Japan.
Dent Traumatol. 2017 Oct;33(5):383-392. doi: 10.1111/edt.12351. Epub 2017 Jul 6.
BACKGROUND/AIMS: An easily available tooth storage medium is required to preserve a tooth after avulsion. Milk and Hank's balanced salt solution (HBSS) are recommended as tooth storage media, and egg white is also reported to be comparable with milk. The aim of this study was to histologically and immunohistochemically evaluate the effect of different tooth storage media on the periodontal ligament (PDL) of extracted teeth.
This experiment used HBSS, milk, and egg white as tooth storage media. A total of ninety-six 6-week-old male Sprague-Dawley rats were used in these experiments. In each experiment, six rats were used for each medium and for the control group. Extracted rat molar teeth were immersed in these three different storage media for 1 hour. In each medium, six samples (n=18) were fixed immediately, and the remaining samples (n=54) were subcutaneously transplanted. In the control group (n=24), the extracted teeth were fixed or transplanted immediately after extraction. At day 4, 1 and 2 weeks after transplantation, the teeth were examined by radiographic, histological, and immunohistochemical methods. The number of PDL cells in the storage media was also counted.
Teeth immersed for 1 hour in milk showed the thinnest PDL. Immunohistochemistry of periostin and CD68 labeling suggested degradation of the extracellular matrix in the PDL. In the media used for immersion, more PDL cells were observed in milk than in the other solutions. After transplantation, the HBSS and egg white groups maintained adequate thickness of PDL but in the milk group, thinner PDL and ankylosis were observed.
Adequate thickness of PDL was maintained in the egg white group, whereas the milk group showed disturbance in the PDL, which may lead to ankylosis.
背景/目的：牙齿脱落后需要一种易于获取的牙齿储存介质来保存牙齿。牛奶和汉克平衡盐溶液（HBSS）被推荐作为牙齿储存介质，并且据报道蛋清与牛奶具有相似的效果。本研究的目的是通过组织学和免疫组织化学方法评估不同牙齿储存介质对拔除牙齿牙周膜（PDL）的影响。
本实验使用HBSS、牛奶和蛋清作为牙齿储存介质。这些实验共使用了96只6周龄的雄性Sprague-Dawley大鼠。在每个实验中，每种介质和对照组各使用6只大鼠。将拔除的大鼠磨牙浸入这三种不同的储存介质中1小时。在每种介质中，立即固定6个样本（n = 18），其余样本（n = 54）进行皮下移植。在对照组（n = 24）中，拔除的牙齿在拔除后立即固定或移植。在移植后第4天、1周和2周，通过影像学、组织学和免疫组织化学方法检查牙齿。还对储存介质中的PDL细胞数量进行了计数。
在牛奶中浸泡1小时的牙齿显示出最薄的PDL。骨膜蛋白和CD68标记的免疫组织化学表明PDL中细胞外基质的降解。在用于浸泡的介质中，牛奶中观察到的PDL细胞比其他溶液中更多。移植后，HBSS和蛋清组维持了足够的PDL厚度，但在牛奶组中，观察到PDL更薄且出现了牙根粘连。
蛋清组维持了足够的PDL厚度，而牛奶组显示出PDL紊乱，这可能导致牙根粘连。
