一种用于脱落牙齿的新型存储介质。

A new storage medium for an avulsed tooth.

作者信息

Khademi Abbas Ali, Saei Saeed, Mohajeri Mohammad Reza, Mirkheshti Nooshin, Ghassami Fatima, Torabi nia Nakisa, Alavi Sayyed Ali

机构信息

Department of Endodontics, Isfahan University, Iran.

出版信息

J Contemp Dent Pract. 2008 Sep 1;9(6):25-32.

PMID:18784856

Abstract

AIM

The purpose of this study is to determine the efficacy of egg white in maintaining the viability of human periodontal ligament (PDL) cells on avulsed teeth.

METHODS AND MATERIALS

The experimental media were: egg white, milk, Hanks' Balanced Salt Solution (HBSS) as the positive control, and tap water as the negative control. The storage times were 1, 2, 4, 8, and 12 hours. Extracted premolar teeth of healthy individuals were rinsed in the media. After trypsinization and subsequent treatment in collagenase, cell viability was determined using trypan blue staining. The two-way analysis of variance (ANOVA) statistical test was used to compare the results among different media.

RESULTS

There was no difference in the cell viability between egg white and HBSS media, but there was a statistically significant difference between the viability of PDL cells in egg white medium in comparison with milk (P<0.05) and water (P<0.05).

CONCLUSION

Egg white could be suggested as a suitable storage medium. Its principle advantage is its availability.

摘要

目的

本研究旨在确定蛋清在维持脱位牙上人牙周膜（PDL）细胞活力方面的效果。

方法和材料

实验培养基为：蛋清、牛奶、作为阳性对照的汉克斯平衡盐溶液（HBSS）以及作为阴性对照的自来水。储存时间为1、2、4、8和12小时。将健康个体拔除的前磨牙在培养基中冲洗。经胰蛋白酶消化并随后用胶原酶处理后，使用台盼蓝染色法测定细胞活力。采用双向方差分析（ANOVA）统计检验来比较不同培养基之间的结果。