Wang Jing-Hua, Kim Bong-Soo, Han Kyungsun, Kim Hojun
Department of Rehabilitation Medicine of Korean Medicine, Dongguk University, 814 Siksa, Goyang, Gyeonggi-do 10326, Korea.
Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Traditional Chinese Medicine, Meishan Road 103, Hefei 230038, China.
Int J Environ Res Public Health. 2017 May 23;14(6):555. doi: 10.3390/ijerph14060555.
Changes in gut microbiota (GM) are closely associated with metabolic syndrome, obesity, type 2 diabetes and so on. Several medicinal herbs, including (Es), have anti-obesity effects that ameliorate metabolic disorders. Therefore, in this study we evaluated whether Es maintains its anti-obesity effect through Es-altered gut microbiota (EsM) transplantation. GM was isolated from cecal contents of Es treated and untreated rats following repeated transplants into obese rats via oral gavage over three weeks. High-fat-diet (HFD)-induced obese rats transplanted with EsM lost significant body weight, epididymal fat, and perirenal fat weight, but no remarkable changes were observed in abdominal fat, liver, cecum weight and food efficiency ratio. In addition, treatment with EsM also significantly lowered the fasting blood glucose, serum insulin level, and insulin resistance index. Meanwhile, EsM transplantation significantly reduced gene expression of proinflammatory cytokines interleukin-1 and monocyte chemotactic protein-1. Rats treated with EsM also showed changed GM composition, especially blautia, roseburia and clostridium, significantly reduced the level of endotoxin and markedly increased the acetic acid in feces. Overall, our results demonstrated that EsM ameliorates HFD-induced obesity and related metabolic disorders, like hyperglycemia and insulin resistance, and is strongly associated with modulating the distribution of GM, enterogenous endotoxin and enteral acetic acid.
肠道微生物群（GM）的变化与代谢综合征、肥胖症、2型糖尿病等密切相关。包括（此处原文缺失草药名称）在内的几种草药具有改善代谢紊乱的抗肥胖作用。因此，在本研究中，我们评估了（此处原文缺失草药名称）是否通过（此处原文缺失草药名称）改变的肠道微生物群（EsM）移植来维持其抗肥胖作用。在三周内通过口服灌胃将经（此处原文缺失草药名称）处理和未处理的大鼠盲肠内容物反复移植到肥胖大鼠体内后，从其中分离出肠道微生物群。移植了EsM的高脂饮食（HFD）诱导的肥胖大鼠体重、附睾脂肪和肾周脂肪重量显著减轻，但腹部脂肪、肝脏、盲肠重量和食物效率比未观察到明显变化。此外，EsM治疗还显著降低了空腹血糖、血清胰岛素水平和胰岛素抵抗指数。同时，EsM移植显著降低了促炎细胞因子白细胞介素-1和单核细胞趋化蛋白-1的基因表达。接受EsM治疗的大鼠肠道微生物群组成也发生了变化，尤其是布劳特氏菌属、罗氏菌属和梭菌属，粪便中内毒素水平显著降低，乙酸含量显著增加。总体而言，我们的结果表明，EsM改善了HFD诱导的肥胖症及相关代谢紊乱，如高血糖和胰岛素抵抗，并且与调节肠道微生物群分布、肠源性内毒素和肠道乙酸密切相关。
