Dong Hao, Zhang Feng Jiang, Wang Fu Yuan, Wang Ying Ying, Guo Jing, Kanhar G M, Chen Jing, Liu Jun, Zhou Chen, Yan Min, Chen Xing
Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education of China, Zhejiang University, Hangzhou, Zhejiang, China.
Department of Anesthesia, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
J Chromatogr A. 2017 Jul 14;1506:93-100. doi: 10.1016/j.chroma.2017.05.001. Epub 2017 May 2.
In balanced anesthesia, sevoflurane and propofol are often used in combination to achieve a better anesthetic effect. However, methods for on-line monitoring of concentrations of the two anesthetics in patients are still rare in clinical. This study proposed a non-invasive method utilizing a fast gas chromatograph combined with a surface acoustic wave sensor (Fast GC-SAW) to simultaneously on-line monitor sevoflurane and propofol in patients' exhaled gas. By using the direct resistive heating capillary column, the single detection time of Fast GC-SAW system was significantly shortened to 90s, as well as the size reduced to (40cm×30cm×20cm). Besides, in the calibration of sevoflurane, Fast GC-SAW system showed a good linear correlation (R=0.9925, P<0.01) with gas chromatography-mass spectrometer (GC-MS), which ensured the reliability and accuracy of the Fast GC-SAW system. Finally, clinical experiments on patients under balanced anesthesia were conducted. The varied concentrations measured by Fast GC-SAW extraordinarily matched the clinical usages of these two anesthetics.
在平衡麻醉中，七氟醚和丙泊酚常联合使用以达到更好的麻醉效果。然而，临床上仍缺乏对患者体内这两种麻醉剂浓度进行在线监测的方法。本研究提出了一种利用快速气相色谱仪结合表面声波传感器（Fast GC-SAW）的非侵入性方法，用于同时在线监测患者呼出气体中的七氟醚和丙泊酚。通过使用直接电阻加热毛细管柱，Fast GC-SAW系统的单次检测时间显著缩短至90秒，体积也减小到（40cm×30cm×20cm）。此外，在七氟醚的校准中，Fast GC-SAW系统与气相色谱-质谱联用仪（GC-MS）显示出良好的线性相关性（R = 0.9925，P < 0.01），这确保了Fast GC-SAW系统的可靠性和准确性。最后，对接受平衡麻醉的患者进行了临床实验。Fast GC-SAW测得的变化浓度与这两种麻醉剂的临床使用情况非常匹配。
