Zhang Fengjiang, Dong Hao, Zhang Xuanlang, Guo Jin, Liu Yunqing, Zhou Cheng, Zhang Xi, Liu Jun, Yan Min, Chen Xing
Department of Anesthesia, The Second Affiliated Hospital Zhejiang University School of Medicine, Zhejiang University.
Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education of China, Zhejiang University.
Anal Sci. 2017;33(11):1271-1277. doi: 10.2116/analsci.33.1271.
Propofol (2,6-diisopropylphenol) is widely used in total intravenous anesthesia. An unknown drug concentration in blood always leads to some side effects in patients with propofol injection. However, the drug concentration in the blood is hard to be continuously measured since invasive sampling causes a loss of blood at each measurement. Here, we introduced a virtual surface acoustic wave sensor array (VSAWSA) to non-invasively detect the propofol concentration in blood through exhaled gases. Calibration was conducted by a parallel test using gas chromatography and mass spectrometry (GC-MS) with solid-phase micro-extraction (SPME) for preconcentration. The limit of detection of VSAWSA reached 0.15 nmol/L for propofol. Six cases of clinical trials was conducted to compare the exhaled propofol concentrations to the plasma concentrations controlled by target-controlled infusion (TCI). The calibration by GC-MS ensured the feasibility, reliability, and accuracy of the VSAWSA (R = 0.9904, p <0.001). The clinical monitoring data by VSAWSA showed an excellent consistency with TCI.
丙泊酚（2,6 - 二异丙基苯酚）广泛应用于全静脉麻醉。丙泊酚注射患者血液中未知的药物浓度总会导致一些副作用。然而，由于侵入性采样在每次测量时都会导致失血，血液中的药物浓度难以连续测量。在此，我们引入了一种虚拟表面声波传感器阵列（VSAWSA），通过呼出气体非侵入性地检测血液中的丙泊酚浓度。使用气相色谱 - 质谱联用仪（GC - MS）结合固相微萃取（SPME）进行预浓缩的平行试验进行校准。VSAWSA对丙泊酚的检测限达到0.15 nmol/L。进行了6例临床试验，以比较呼出的丙泊酚浓度与靶控输注（TCI）控制的血浆浓度。GC - MS校准确保了VSAWSA的可行性、可靠性和准确性（R = 0.9904，p <0.001）。VSAWSA的临床监测数据与TCI显示出极好的一致性。
