利用微孔筛选和表面等离子体共振成像技术分离用于蛋白质治疗的单细胞

Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging.

作者信息

Abali F, Stevens M, Tibbe A G J, Terstappen L W M M, van der Velde P N, Schasfoort R B M

机构信息

Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, PO Box 217, 7500AE Enschede, The Netherlands.

VyCAP, Abraham Rademakerstraat 41, 7425PG Deventer, The Netherlands.

出版信息

Anal Biochem. 2017 Aug 15;531:45-47. doi: 10.1016/j.ab.2017.05.021. Epub 2017 May 22.

DOI:10.1016/j.ab.2017.05.021

PMID:28545866

Abstract

Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

摘要

在此证明了通过结合表面等离子体共振成像（SPRi）和自分选微孔技术来监测单个细胞的产物分泌的可行性。此外，可以利用通过SPRi获得的微孔位置坐标从微孔中冲压出细胞，从而对选定的细胞进行分离。可以从微孔阵列中无菌回收感兴趣的细胞以进行进一步培养。