Medical Cell BioPhysics Group (MCBP), University of Twente, 7500 AE Enschede, The Netherlands.
Interfluidics BV, 7483 AL Haaksbergen, The Netherlands.
Biosensors (Basel). 2018 Oct 30;8(4):102. doi: 10.3390/bios8040102.
SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.
SPR 细胞仪使用表面等离子体共振 (SPR) 现象来测量完整细胞的参数。SPR 成像 (SPRi) 仪器的出现使得对活细胞与传感器表面的特异性实时无标记结合成为可能，研究人员在过去十年中开始探索其潜力。在这里，我们将讨论检测机制，并根据我们自己的经验和文献信息，另外描述哺乳动物细胞在 SPR 生物传感中的问题和挑战。最后，我们将基于该领域已经实现的知识和应用，对 SPRi 细胞仪的一些令人兴奋的应用进行预测。
