Cotte Christina, Szczepanek Steven M
Center of Excellence for Vaccine Research, Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, United States.
Center of Excellence for Vaccine Research, Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, United States.
Vaccine. 2017 Jun 16;35(28):3520-3522. doi: 10.1016/j.vaccine.2017.05.039. Epub 2017 May 22.
Long-term immunity after inoculation with the pneumococcal conjugate vaccine (Prevnar-13) is impaired in sickle cell disease (SCD) mice. We sought to determine which B-cell subsets are defective in SCD mice after vaccination with Prevnar-13, yet confer long-term immunity in wild-type (WT) mice. We vaccinated WT and SCD mice three times at three week intervals with Prevnar-13. Fourteen weeks later, 5∗10 cells of isolated peritoneal B-1a, B-1b, and B-2 cells were harvested and intraperitoneally transferred to Rag -/- recipients. A week later recipients were intraperitoneally challenged with 10CFU of Streptococcus pneumoniae (serotype 3). Recipient mice that received either B-1b or B-2 B-cells from WT mice survived challenge, whereas mice that received B-1a cells died. Recipient mice that received B-1a, B-1b, or B-2 cells from SCD mice died after challenge. Both B-1b and B-2 cells appear to confer long-term immunity after Prevnar-13 vaccination, yet neither subset functions properly in SCD mice.
接种肺炎球菌结合疫苗（沛儿13）后，镰状细胞病（SCD）小鼠的长期免疫力受损。我们试图确定在接种沛儿13后，SCD小鼠中哪些B细胞亚群存在缺陷，而这些亚群在野生型（WT）小鼠中能赋予长期免疫力。我们以三周的间隔给WT和SCD小鼠接种三次沛儿13。十四周后，收集分离出的腹膜B-1a、B-1b和B-2细胞，取5×10个细胞腹腔注射到Rag -/-受体小鼠体内。一周后，给受体小鼠腹腔注射10CFU的肺炎链球菌（血清型3）进行攻毒。接受来自WT小鼠的B-1b或B-2 B细胞的受体小鼠在攻毒后存活，而接受B-1a细胞的小鼠死亡。接受来自SCD小鼠的B-1a、B-1b或B-2细胞的受体小鼠在攻毒后死亡。B-1b和B-2细胞在接种沛儿13后似乎都能赋予长期免疫力，但在SCD小鼠中这两个亚群均不能正常发挥功能。
