Soria-Lozano P, Gilaberte Y, Paz-Cristobal M P, Pérez-Artiaga L, Lampaya-Pérez V, Aporta J, Pérez-Laguna V, García-Luque I, Revillo M J, Rezusta A
Department of Microbiology, Hospital Universitario Miguel Servet, Zaragoza, Spain.
Department of Dermatology, Hospital San Jorge, Huesca, Spain.
BMC Microbiol. 2015 Sep 26;15:187. doi: 10.1186/s12866-015-0524-3.
Antimicrobial photodynamic therapy has been proposed as an alternative to suppress subgingival species. This results from the balance among Streptococcus sanguis, Streptococcus mutans and Candida albicans in the dental biofilm. Not all the photosensitizers have the same photodynamic effect against the different microorganims. The objective of this study is to compare in vitro the photodynamic effect of methylene blue (MB), rose Bengal (RB) and curcumin (CUR) in combination with white light on the cariogenic microorganism S. mutans, S. sanguis and C. albicans.
Photodynamic therapy with MB, RB and CUR inhibited 6 log 10 the growth of both bacteria but at different concentrations: 0.31-0.62 μg/ml and 0.62-1.25 μg/ml RB were needed to photoinactivate S. mutans and S. sanguis, respectively; 1.25-2.5 μg/ml MB for both species; whereas higher CUR concentrations (80-160 μg/ml and 160-320 μg/ml) were required to obtain the same reduction in S. mutans and S. sanguis viability respectively. The minimal fungicidal concentration of MB for 5 log10 CFU reduction (4.5 McFarland) was 80-160 μg/ml, whereas for RB it ranged between 320 and 640 μg/ml. For CUR, even the maximum studied concentration (1280 μg/ml) did not reach that inhibition. Incubation time had no effect in all experiments.
Photodynamic therapy with RB, MB and CUR and white light is effective in killing S. mutans and S. sanguis strains, although MB and RB are more efficient than CUR. C. albicans required higher concentrations of all photosensitizers to obtain a fungicidal effect, being MB the most efficient and CUR ineffective.
抗菌光动力疗法已被提议作为抑制龈下菌群的一种替代方法。这是由牙菌斑中血链球菌、变形链球菌和白色念珠菌之间的平衡导致的。并非所有光敏剂对不同微生物都具有相同的光动力效应。本研究的目的是在体外比较亚甲蓝（MB）、孟加拉玫瑰红（RB）和姜黄素（CUR）与白光联合对致龋微生物变形链球菌、血链球菌和白色念珠菌的光动力效应。
MB、RB和CUR的光动力疗法均抑制了两种细菌6个对数级的生长，但所需浓度不同：分别需要0.31 - 0.62μg/ml和0.62 - 1.25μg/ml的RB来使变形链球菌和血链球菌光灭活；两种菌均需要1.25 - 2.5μg/ml的MB；而分别需要更高浓度的CUR（80 - 160μg/ml和160 - 320μg/ml）才能使变形链球菌和血链球菌的活力有相同程度的降低。使5个对数级CFU减少（4.5麦氏浊度）的MB的最低杀菌浓度为80 - 160μg/ml，而RB的最低杀菌浓度在320至640μg/ml之间。对于CUR，即使是研究的最高浓度（1280μg/ml）也未达到那种抑制效果。在所有实验中孵育时间均无影响。
RB、MB和CUR与白光联合的光动力疗法对杀死变形链球菌和血链球菌菌株有效，尽管MB和RB比CUR更有效。白色念珠菌需要所有光敏剂的更高浓度才能获得杀菌效果，其中MB最有效，CUR无效。
