Watanabe K, Takanashi K, Yoshizawa I
Hokkaido Institute of Pharmaceutical Sciences, Japan.
Steroids. 1988 Jul-Aug;52(1-2):123-36. doi: 10.1016/0039-128x(88)90221-8.
The measurement of urinary estradiol-17-sulfate concentration by direct radioimmunoassay was established. The urinary estradiol-17-sulfate levels measured by the radioimmunoassay correlated well with those determined by high-performance liquid chromatography equipped with electrochemical detector. Estradiol-17-sulfate concentrations in early morning urine of six healthy adult men was 8.2 +/- 2.0 ng/mL, or 5.7 +/- 1.8 ng/mg creatinine. The urinary levels in women throughout the menstrual cycle showed a characteristic three-peak excretion pattern: the first and the second peaks appeared just after and three days before the urinary LH peak, and the third peak appeared a few days before menstruation.
建立了通过直接放射免疫测定法测量尿中雌二醇 - 17 - 硫酸盐浓度的方法。通过放射免疫测定法测得的尿雌二醇 - 17 - 硫酸盐水平与配备电化学检测器的高效液相色谱法测定的结果具有良好的相关性。6名健康成年男性晨尿中的雌二醇 - 17 - 硫酸盐浓度为8.2±2.0 ng/mL，或5.7±1.8 ng/mg肌酐。女性在整个月经周期中的尿水平呈现出特征性的三峰排泄模式：第一个和第二个峰分别出现在尿促黄体生成素（LH）峰之后和之前三天，第三个峰出现在月经前几天。
