Caporossi D, Vernole P, Porfirio B, Tedeschi B, Frezza D, Nicoletti B, Calef E
Department of Public Health and Cell Biology, 2nd University of Rome, Italy.
Cytogenet Cell Genet. 1988;48(4):220-3. doi: 10.1159/000132632.
Localization of Epstein-Barr virus (EBV) DNA was studied by in situ hybridization on chromosomes from the Namalwa Burkitt lymphoma cell line and from a lymphoblastoid cell line transformed in vitro (ATL9/g). The five chromosome bands 1p32, 1q31, 5q21, 13q21, and 16p13 showed the presence of EBV DNA in both of the lines. Grain deposition at the site on chromosome 1q of the Burkitt line was particularly intense. It was also found that EBV DNA in the lymphoblastoid cell line co-localized with a stable achromatic gap at 1p32 whose presence seems to confer a proliferative advantage on the cells.
通过原位杂交技术，对来自纳马勒瓦伯基特淋巴瘤细胞系以及体外转化的淋巴母细胞系（ATL9/g）的染色体进行研究，以确定爱泼斯坦-巴尔病毒（EBV）DNA的定位。在这两个细胞系中，1p32、1q31、5q21、13q21和16p13这五条染色体带均显示存在EBV DNA。伯基特细胞系中，位于1q染色体位点处的颗粒沉积尤为强烈。还发现，淋巴母细胞系中的EBV DNA与1p32处的一个稳定无色间隙共定位，该间隙的存在似乎赋予了细胞增殖优势。
