Henderson A, Ripley S, Heller M, Kieff E
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1987-91. doi: 10.1073/pnas.80.7.1987.
The Epstein-Barr virus (EBV) genome stably persists in latently infected Burkitt tumor cells and growth-transformed B lymphocytes. These cells usually contain multiple copies of episomal viral DNA. Cytological hybridization of recombinant viral DNA fragments to metaphase chromosomes of two latently infected cell lines demonstrates that viral DNA localizes to both chromatids of one homologue of chromosome 1 in Namalwa, a Burkitt tumor cell line, and to both chromatids of one homologue of chromosome 4 in IB4, a cell line with transformed growth properties in vitro. The site of chromosome association remains stable in a clone of IB4 cells. Probes from five separate regions of the EBV genome hybridize to the same chromosome regions. A previously undescribed achromatic site is identified within the region of EBV chromosome cytological hybridization. These observations suggest that most or all of the EBV genome is integrated into the chromosomal DNA of Namalwa and IB4 cells. Although the chromosomal sites of EBV DNA association are among those regions with homology to the EBV IR3 repeated DNA sequence, EBV IR3 did not mediate recombination between EBV and chromosomal DNA.
爱泼斯坦-巴尔病毒（EBV）基因组稳定存在于潜伏感染的伯基特肿瘤细胞和生长转化的B淋巴细胞中。这些细胞通常含有多个附加型病毒DNA拷贝。重组病毒DNA片段与两个潜伏感染细胞系的中期染色体进行细胞学杂交表明，在伯基特肿瘤细胞系Namalwa中，病毒DNA定位于1号染色体一个同源染色体的两条染色单体上，而在体外具有转化生长特性的细胞系IB4中，病毒DNA定位于4号染色体一个同源染色体的两条染色单体上。染色体关联位点在IB4细胞克隆中保持稳定。来自EBV基因组五个不同区域的探针与相同的染色体区域杂交。在EBV染色体细胞学杂交区域内鉴定出一个以前未描述的无色区域。这些观察结果表明，大部分或所有EBV基因组已整合到Namalwa和IB4细胞的染色体DNA中。尽管EBV DNA关联的染色体位点位于与EBV IR3重复DNA序列具有同源性的区域中，但EBV IR3并未介导EBV与染色体DNA之间的重组。
