Nagase Kenichi, Nagumo Yuhei, Kim Miri, Kim Hee-Jung, Kyung Hei-Won, Chung Hye-Jin, Sekine Hidekazu, Shimizu Tatsuya, Kanazawa Hideko, Okano Teruo, Lee Seung-Jin, Yamato Masayuki
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawadacho, Shinjuku, Tokyo, 162-8666, Japan.
Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan.
Macromol Biosci. 2017 Aug;17(8). doi: 10.1002/mabi.201700073. Epub 2017 May 26.
Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF-releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF-releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications.
细胞片移植是一项关键的组织工程技术。一种用于多层心肌细胞片移植的可释放血管内皮生长因子（VEGF）的纤维垫被研发出来。通过静电纺丝和电喷雾法制备了负载含有VEGF的聚（乳酸-乙醇酸）纳米颗粒的聚乙烯醇纤维垫。将带有可释放VEGF垫的六层心肌细胞片移植到无胸腺大鼠体内。两周后，与没有可释放VEGF垫的对照组相比，这些细胞片产生了更厚的心肌细胞层，并整合了含有红细胞的更大直径血管。因此，移植的心肌细胞附近局部释放VEGF可诱导血管生成，从而提供足够的氧气和营养物质以防止坏死。相比之下，没有可释放VEGF垫的心肌细胞片在体内无法存活，可能会发生坏死，并且厚度会减小。因此，这些可释放VEGF的垫能够在单一程序中实现多层心肌细胞片的移植，并应扩大细胞片移植在治疗应用中的潜力。
