Mohammad Khanlou Ziba, Pouladi Nasser, Hosseinpour Feizi Mohammadali, Pedram Negar
Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. Email:
Asian Pac J Cancer Prev. 2017 Apr 1;18(4):1133-1138. doi: 10.22034/APJCP.2017.18.4.1133.
Background and Aim: MDM4, a negative regulator of the p53 tumor suppression pathway, has been demonstrated to be overexpressed in a variety of human cancers. Research has revealed that the rs4245739 A>C polymorphism of MDM4 in the 3′-untranslated region makes it a miR-191 target site, leading to lower MDM4 expression. This study aimed to detect if the rs4245739 single nucleotide polymorphism (SNP) impacts on thyroid cancer (TC) development in Iranian-Azeri patients. Materials and Method: Blood samples were taken from 232 healthy controls and 130 TC patients of Iranian-Azeri ethnicity. For genotyping, Tetra-ARMS PCR was performed. SPSS for Windows (version 22.0, IBM SPSS Inc., USA) and the SHEsis online software were used for data analysis. Results: Alleles of MDM4 rs4245739 SNP demonstrated no significant different in frequencies between patients and controls (p>0.05). Additionally, genotypes of MDM4 rs4245739 SNP did not increase or decrease TC risk in patients compared with healthy subjects. Conclusion: Considering the lack of any observed association between the MDM4 rs4245739 polymorphism and TC, we conclude no significant role in the pathophysiology of the disease.
MDM4是p53肿瘤抑制通路的负调节因子，已证实在多种人类癌症中过度表达。研究表明，MDM4基因3′-非翻译区的rs4245739 A>C多态性使其成为miR-191的靶位点，导致MDM4表达降低。本研究旨在检测rs4245739单核苷酸多态性（SNP）是否影响伊朗阿塞拜疆族患者的甲状腺癌（TC）发生。材料与方法：采集232名健康对照者和130名伊朗阿塞拜疆族TC患者的血样。采用四引物扩增受阻突变系统PCR进行基因分型。使用SPSS for Windows（版本22.0，IBM SPSS公司，美国）和SHEsis在线软件进行数据分析。结果：MDM4 rs4245739 SNP的等位基因在患者和对照者中的频率无显著差异（p>0.05）。此外，与健康受试者相比，MDM4 rs4245739 SNP的基因型并未增加或降低患者的TC风险。结论：鉴于未观察到MDM4 rs4245739多态性与TC之间存在任何关联，我们得出其在该疾病病理生理学中无显著作用的结论。
