Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou 310016, China.
ACS Appl Mater Interfaces. 2017 Jun 14;9(23):19698-19705. doi: 10.1021/acsami.7b05296. Epub 2017 Jun 5.
Two important goals in orthopedic implant research are to promote osseointegration and prevent infection. However, much previous effort has been focused on the design of coatings to either enhance osseointegration while ignoring antibacterial activity or vice versa, to prevent infection while ignoring bone integration. Here, we designed a multifunctional mineralized collagen coating on titanium with the aid of metal-organic framework (MOF) nanocrystals to control the release of naringin, a Chinese herbal medicine that could promote osseointegration and prevent bacterial infection. The attachment, proliferation, osteogenic differentiation, and mineralization of mesenchymal stem cells on the coating were significantly enhanced. Meanwhile, the antibacterial abilities against Staphylococcus aureus were also promoted. Furthermore, release kinetics analysis indicated that the synergistic effect of a primary burst release stage and secondary slow release stage played a critical role in the performance and could be controlled by the relative concentrations of MOF and naringin. This work thus provides a novel strategy to engineer multifunctional orthopedic coatings that can enhance osseointegration and simultaneously inhibit microbial cell growth.
在骨科植入物研究中，有两个重要目标，即促进骨整合和预防感染。然而，以前的许多研究都集中在设计涂层上，要么增强骨整合而忽略抗菌活性，要么相反，为了预防感染而忽略了骨整合。在这里，我们在钛金属上设计了一种具有多功能矿化胶原涂层，借助金属有机骨架（MOF）纳米晶体来控制柚皮苷的释放，柚皮苷是一种中草药，可促进骨整合并预防细菌感染。涂层上的间充质干细胞的附着、增殖、成骨分化和矿化明显增强。同时，对金黄色葡萄球菌的抗菌能力也得到了提高。此外，释放动力学分析表明，初始突释阶段和二次缓慢释放阶段的协同作用在性能中起着关键作用，并且可以通过 MOF 和柚皮苷的相对浓度来控制。这项工作为工程多功能骨科涂层提供了一种新策略，可增强骨整合并同时抑制微生物细胞生长。
