Tang Sheng, Ouyang Zhu, Tan Xiang, Liu Xin, Bai Junying, Wang Hua, Huang Linhua
Citrus Research Institute, Southwest University, Chongqing 400700, China.
National Citrus Engineering Research Center, Chongqing 400700, China.
Foods. 2024 Feb 14;13(4):576. doi: 10.3390/foods13040576.
Naringin is one of the common flavonoids in grapefruit, which has anti-cancer, antioxidant, and anti-inflammatory activities. However, its poor solubility limits its wide application. Therefore, the aim of this study is to investigate the anti-inflammatory effect of naringin combined with chitooligosaccharides with good biocompatibility by constructing a mouse model of systemic inflammatory response syndrome (SIRS). The results showed that the naringin-chitooligosaccharide (NG-COS) complex significantly inhibited lipopolysaccharide (LPS)-induced weight loss, reduced food intake, tissue inflammatory infiltration, and proinflammatory cytokines IL-6, TNF-α, INF-γ, and IL-1β levels. The complex also significantly affected the content of malondialdehyde and the activities of MPO, SOD, and GSH in the liver, spleen, lungs, and serum of mice with systemic inflammation. In addition, NG-COS significantly inhibited the mRNA expression of inflammatory factors in the TLR4/NF-κB signaling pathway. Principal component analysis showed that the complexes could inhibit LPS-induced systemic inflammation in mice, and the effect was significantly better than that of naringin and chitooligosaccharides alone. This study explored the synergistic effects of chitosan and naringin in reducing inflammation and could contribute to the development of novel biomedical interventions.
柚皮苷是葡萄柚中常见的黄酮类化合物之一，具有抗癌、抗氧化和抗炎活性。然而，其溶解性差限制了其广泛应用。因此，本研究的目的是通过构建全身炎症反应综合征（SIRS）小鼠模型，研究柚皮苷与具有良好生物相容性的壳寡糖联合使用的抗炎效果。结果表明，柚皮苷 - 壳寡糖（NG - COS）复合物显著抑制脂多糖（LPS）诱导的体重减轻、食物摄入量减少、组织炎症浸润以及促炎细胞因子IL - 6、TNF - α、INF - γ和IL - 1β水平。该复合物还显著影响全身炎症小鼠肝脏、脾脏、肺脏和血清中丙二醛的含量以及MPO、SOD和GSH的活性。此外，NG - COS显著抑制TLR4/NF - κB信号通路中炎症因子的mRNA表达。主成分分析表明，该复合物可抑制LPS诱导的小鼠全身炎症，且效果明显优于单独使用柚皮苷和壳寡糖。本研究探讨了壳聚糖和柚皮苷在减轻炎症方面的协同作用，可为新型生物医学干预措施的开发做出贡献。
