Zhao Xiao, Xu Youfa, Zhang Lifang, Wang Chunmei, Guo Chunna, Fei Chenzhong, Zhang Keyu, Wang Xiaoyang, Liu Yingchun, Wang Mi, Zheng Wenli, Xue Feiqun
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
China College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou 510642, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Aug 1;1059:1-6. doi: 10.1016/j.jchromb.2017.04.049. Epub 2017 May 11.
Ethanamizuril (EZL) is a novel anti-coccidial triazine synthesized by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences. As a novel coccidiostat, it is essential to study its residue in food animal tissues. In this study, a reliable UPLC method for the determination of ethanamizuril (EZL) and one of its metabolites (M3) in chicken muscle, skin and fat, liver, and kidney samples was developed. Analytes were extracted using acetonitrile and 10% sodium carbonate solution, defatted by n-hexane, and further purified by using an Oasis MCX cartridge. The calibration curves for both test compounds were linear in all tissue matrices we tested: muscle, skin and fat, liver and kidney. The mean relative recoveries of EZL and M3 were from 87.3% to 103.2% between all tissue samples. The inter-day and intra-day relative standard deviations were within 15% and 20%, respectively. This method is accurate, reproducible and is ready to be field-tested.
乙胺嘧啶（EZL）是中国农业科学院上海兽医研究所合成的一种新型抗球虫三嗪。作为一种新型抗球虫药，研究其在食用动物组织中的残留至关重要。本研究建立了一种可靠的超高效液相色谱法，用于测定鸡肉、皮肤和脂肪、肝脏和肾脏样品中的乙胺嘧啶（EZL）及其一种代谢物（M3）。分析物用乙腈和10%碳酸钠溶液提取，用正己烷脱脂，再用Oasis MCX柱进一步净化。两种被测化合物的校准曲线在我们测试的所有组织基质（肌肉、皮肤和脂肪、肝脏和肾脏）中均呈线性。在所有组织样品中，EZL和M3的平均相对回收率在87.3%至103.2%之间。日间和日内相对标准偏差分别在15%和20%以内。该方法准确、可重复，可随时进行现场测试。
