超高效液相色谱串联质谱法测定猪和鸡组织中维吉尼亚霉素 M1 残留

Determination of virginiamycin M1 residue in tissues of swine and chicken by ultra-performance liquid chromatography tandem mass spectrometry.

机构信息

Key Laboratory of Veterinary Chemical Drug and Pharmaceutics, Ministry of Agriculture, Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China; Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.

出版信息

Food Chem. 2018 Jun 1;250:127-133. doi: 10.1016/j.foodchem.2018.01.024. Epub 2018 Jan 3.

DOI:10.1016/j.foodchem.2018.01.024

PMID:29412901

Abstract

A reliable UPLC-MS/MS method with high sensitivity was developed and validated for the determination of virginiamycin M1 in muscle, fat, liver, and kidney samples of chicken and swine. Analytes were extracted using acetonitrile and extracts were defatted by N-hexane. Chromatographic separation was performed on a BEH C18 liquid chromatography column. The analytes were then detected using triplequadrupole mass spectrometry in positive electrospray ionization and multiple reaction monitoring mode. Calibration plots were constructed using standard working solutions and showed good linearity. Limits of quantification ranged from 2 to 60 ng mL.

摘要

建立并验证了一种灵敏可靠的 UPLC-MS/MS 方法，用于测定鸡肉、猪肉中肌肉、脂肪、肝脏和肾脏组织中的维吉尼亚霉素 M1。样品用乙腈提取，用正己烷脱脂。采用 BEH C18 液相色谱柱进行色谱分离，在正电喷雾电离和多反应监测模式下，用三重四极杆质谱仪进行检测。采用标准工作溶液绘制校准曲线，线性良好。定量下限为 2 至 60ng/mL。