Department of Chemistry, Shahreza Branch, Islamic Azad University, P.O. Box 311-86145, Shahreza, Isfahan, Iran; Young Researchers and Elite Club, Shahreza Branch, Islamic Azad University, Shahreza, Iran.
Department of Chemistry, Shahreza Branch, Islamic Azad University, P.O. Box 311-86145, Shahreza, Isfahan, Iran; Young Researchers and Elite Club, Shahreza Branch, Islamic Azad University, Shahreza, Iran; Razi Chemistry Research Center (RCRC), Shahreza Branch, Islamic Azad University, Isfahan, Iran.
J Colloid Interface Sci. 2017 Oct 15;504:186-196. doi: 10.1016/j.jcis.2017.05.049. Epub 2017 May 19.
A non enzymatic modified electrode was constructed based on the modification of carbon paste electrode with CuO nanoflower (CuO-CPE). The raw and modified samples were characterized by XRD, TEM, SEM-EDX and X-ray mapping techniques. The proposed CuO-CPE showed a well voltammetric peak pair in cyclic voltammetry which of peak currents were decreased in the presence of glucose. Hence, this decrease in peak current was used for voltammetric determination of glucose. To evaluate interactions between the influencing variables, experiments were designed by response surface methodology (RSM) and the results showed CuO%-pH have the most effect on square wave voltammetric response. The best response was obtained in a run including 20% CuO modifier, pH 3.6, amplitude 0.106V, step potential 0.0074V and frequency 17.75Hz. Calibration curve was constructed and a linear response between ΔIp (difference of peak current in the presence and absence of glucose in SqW voltammograms) and glucose concentration was obtained in concentration range from 0.06 to 10mmolL [calibration equation: ΔI (μA)=91.35 C+523.12, R=0.9967] with detection and quantification limits of 7.49×10 and 2.49×10molL, respectively. The modified electrode can be used satisfactory in determination of glucose in real samples such as human blood serum.
基于氧化铜纳米花（CuO-CPE）修饰碳糊电极构建了一种非酶修饰电极。采用 XRD、TEM、SEM-EDX 和 X 射线映射技术对原始和修饰后的样品进行了表征。在循环伏安法中，所提出的 CuO-CPE 显示出一对良好的伏安峰对，其中在存在葡萄糖的情况下峰电流减小。因此，这种峰电流的减小被用于葡萄糖的伏安测定。为了评估影响变量之间的相互作用，通过响应面法（RSM）设计了实验，结果表明 CuO%-pH 对方波伏安响应的影响最大。在包括 20%CuO 修饰剂、pH3.6、振幅 0.106V、阶跃电位 0.0074V 和频率 17.75Hz 的运行中获得了最佳响应。构建了校准曲线，并在 SqW 伏安图中存在和不存在葡萄糖的峰电流之间的ΔIp（差异）与葡萄糖浓度之间获得了线性响应，在 0.06 至 10mmolL 的浓度范围内[校准方程：ΔI（μA）=91.35C+523.12，R=0.9967]，检测限和定量限分别为 7.49×10 和 2.49×10molL。修饰电极可满意地用于测定人血清等实际样品中的葡萄糖。
