Wang Li, Liu Hua-Min, Qin Guang-Yong
Institute of Physical Science and Engineering, Zhengzhou University, Zhengzhou 450001, China.
College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China.
Food Chem. 2017 Nov 1;234:314-322. doi: 10.1016/j.foodchem.2017.05.002. Epub 2017 May 3.
In the present study, three polysaccharide fractions, QSMP-1, QSMP-2, and QSMP-3, were isolated and purified from the seed meal of Chinese quince. These fractions' structures were investigated by high performance anion exchange chromatography (HPAEC), gel permeation chromatography (GPC), Fourier transform infrared (FT-IR), and nuclear magnetic resonance (NMR), and their antioxidant activities were assessed. The results showed that QSMP-1 is a novel polysaccharide with a backbone mainly composed of →4)-Glcp-(1→, →6)-Glcp-(1→, →6)-Galp-(1→, →2, 3, 4)-Xylp-(1→. The side chains consist of →4)-Arap-(1→, →3, 4)-Arap-(1→, →2)-Galp-(1→, →4)-Manp-(1→, →3)-Galp-(1→, and →3, 6)-Glcp-(1→ with the non-reducing terminals Glcp and Galp. QSMP-1 exhibited effective antioxidant activities by ferrous ion chelation and superoxide anion-scavenging in a dose-dependent manner. These investigations of the polysaccharides from the seed meal of Chinese quince provide a scientific basis for the use of the by-products of quince seed oil processing, particularly as an ingredient in functional foods and medicines.
在本研究中，从木瓜籽粕中分离并纯化出三种多糖组分，即QSMP - 1、QSMP - 2和QSMP - 3。通过高效阴离子交换色谱（HPAEC）、凝胶渗透色谱（GPC）、傅里叶变换红外光谱（FT - IR）和核磁共振（NMR）对这些组分的结构进行了研究，并评估了它们的抗氧化活性。结果表明，QSMP - 1是一种新型多糖，其主链主要由→4)-Glcp-(1→、→6)-Glcp-(1→、→6)-Galp-(1→、→2, 3, 4)-Xylp-(1→组成。侧链由→4)-Arap-(1→、→3, 4)-Arap-(1→、→2)-Galp-(1→、→4)-Manp-(1→、→3)-Galp-(1→和→3, 6)-Glcp-(1→组成，非还原末端为Glcp和Galp。QSMP - 1通过亚铁离子螯合和超氧阴离子清除表现出有效的抗氧化活性，且呈剂量依赖性。这些对木瓜籽粕多糖的研究为木瓜籽油加工副产品的利用提供了科学依据，特别是作为功能性食品和药品的成分。
