Lin Zhier, Liu Sisi, Wang Yi, Chen Jianfang, Huang Jihong, Huang Ruqiang
School of Life Sciences, Guangdong Provincial Engineering Technology Research Center for Drug and Food Biological Resources Processing and Comprehensive Utilization, South China Normal University, Guangzhou 510631, China.
School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Foods. 2024 Nov 25;13(23):3791. doi: 10.3390/foods13233791.
Raspberry ( Hu) is a Chinese herb that is rich in nutrients and has anti-inflammatory, antibacterial, antioxidant, anti-allergic, hypoglycemic, and other effects. A water-soluble polysaccharide was extracted from raspberry by using hot water extraction then purified by DEAE-Sepharose Fast Flow column chromatography. The structural characteristics of the polysaccharide (R1) are as follows: the molar ratio of the monosaccharide composition is Ara:Gal:Xyl:Glc:Man = 31.15:27.64:13.61:13.48:10.60; the molecular weight is 32,580 Da; the methylation results show that 5-Araf is the main chain and there is a presence of 3,6-Galp, 4-Xylp, and 2,3,5-Araf branches, and that terminal Araf (T-Araf) is the major telomeric sugar. It contains α and β glycosidic bonds and is highly branched, with the presence of a helical structure. In the in vitro antioxidant assay, R1 showed the highest scavenging of superoxide anion radicals at 70.38%, followed by the scavenging of DPPH radicals at 52.9% and the scavenging of hydroxyl radicals at 29.28%. In immunomodulation and anti-cancer experiments, R1 did not significantly inhibit or promote RAW264.7 cells but was able to increase the expression of anti-inflammatory cytokines in a concentration-dependent manner. It also significantly inhibited cancer cell survival. R1 enhances immunity by limiting the proliferation of cancer cells primarily through direct inhibition while promoting the secretion of pro-inflammatory cytokines. These findings reveal the potential benefits of raspberry polysaccharides and provide evidence for developing immunologically functional products from raspberry polysaccharides.
树莓（Hu）是一种富含营养的中草药，具有抗炎、抗菌、抗氧化、抗过敏、降血糖等作用。采用热水提取法从树莓中提取水溶性多糖，然后通过DEAE-琼脂糖快速流动柱色谱法进行纯化。该多糖（R1）的结构特征如下：单糖组成的摩尔比为Ara:Gal:Xyl:Glc:Man = 31.15:27.64:13.61:13.48:10.60；分子量为32,580 Da；甲基化结果表明，5-Araf是主链，存在3,6-Galp、4-Xylp和2,3,5-Araf分支，末端Araf（T-Araf）是主要的端粒糖。它含有α和β糖苷键，高度分支，存在螺旋结构。在体外抗氧化试验中，R1对超氧阴离子自由基的清除率最高，为70.38%，其次是对DPPH自由基的清除率为52.9%，对羟基自由基 的清除率为29.28%。在免疫调节和抗癌实验中，R1对RAW264.7细胞没有显著的抑制或促进作用，但能够以浓度依赖的方式增加抗炎细胞因子的表达。它还显著抑制癌细胞的存活。R1主要通过直接抑制癌细胞的增殖来增强免疫力，同时促进促炎细胞因子的分泌。这些发现揭示了树莓多糖的潜在益处，并为开发具有免疫功能的树莓多糖产品提供了证据。
