This descriptive survey study aimed to explore how nursing students perceive spirituality/spiritual care and investigate the variables acting on their perception. Data were collected using the Spirituality and Spiritual Care Rating Scale with 500 students from the Faculty of Nursing. The students' median score regarding their perception of spirituality and spiritual care indicates a "conceptual confusion" related with these concepts. Female students have higher scale scores than male students (z = 2.19, p < 0.05). Students' awareness of spirituality and related concepts, and their acquisition of spiritual care skills will allow them to provide spiritual care after graduation.