Bertucci Juan Ignacio, Tovar Mario Oswaldo, Blanco Ayelén Melisa, Gómez-Requeni Pedro, Unniappan Suraj, Canosa Luis Fabián
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús (IIB-INTECH), Intendente Marino Km 8.2, B7130IWA, Chascomús, Buenos Aires CC 164 (7130), Argentina.
Departamento de Fisiología (Fisiología Animal II), Facultad de Biología, Universidad Complutense de Madrid, Madrid, Spain.
Comp Biochem Physiol B Biochem Mol Biol. 2017 Aug;210:29-38. doi: 10.1016/j.cbpb.2017.05.005. Epub 2017 May 26.
Pejerrey, Odontesthes bonariensis, is an euryhaline fish of commercial importance in Argentina. This work aimed to determine if water salinity affects the expression of genes involved in somatic growth (gh; ghr-I; ghr-II; igf-I), lipid metabolism (Δ6-desaturase) and food intake (nucb2/nesfatin-1). First, we identified the full-length cDNA sequences of Δ6-desaturase (involved in lipid metabolism) and nesfatin-1 (an anorexigen). Then, pejerrey juveniles were reared during 8weeks in three different water salinity conditions: 2.5g/L (S2.5), 15g/L (S15) and 30g/L (S30) of NaCl. Brain, pituitary, liver and muscle samples were collected in order to analyze mRNA expression. The expression of gh and ghr-II mRNAs increased in the pituitary of fish reared at S2.5 and S30 compared with the S15 group. The expression of ghr-I was higher in the liver of S30 group compared to S2.5 and S15. Igf-I mRNA expression in liver increased with the increment of water salinity, while it decreased in the muscle of S15 and S30 groups. Δ6-desaturase expression increased in S2.5 group compared to S15 in both liver and muscle. S30 caused a decrease in the Δ6-desaturase expression in liver compared to S15. The S30 treatment produced an increase in nucb2/nesfatin-1 mRNA expression in the brain and liver compared to S2.5 and S15. The changes in gene expression observed could help pejerrey perform better during salinity challenges. The S30 condition would likely promote pejerrey somatic growth in the long term.
银汉鱼，即博氏半线脂鲤，是一种广盐性鱼类，在阿根廷具有重要的商业价值。这项研究旨在确定水体盐度是否会影响参与体细胞生长（生长激素；生长激素受体-I；生长激素受体-II；胰岛素样生长因子-I）、脂质代谢（Δ6-去饱和酶）和食物摄入（核结合蛋白2/nesfatin-1）的基因表达。首先，我们鉴定了参与脂质代谢的Δ6-去饱和酶和厌食素nesfatin-1的全长cDNA序列。然后，将银汉鱼幼鱼在三种不同的水体盐度条件下饲养8周：2.5g/L（S2.5）、15g/L（S15）和30g/L（S30）的氯化钠。采集脑、垂体、肝脏和肌肉样本以分析mRNA表达。与S15组相比，饲养在S2.5和S30盐度下的鱼垂体中生长激素和生长激素受体-II mRNA的表达增加。与S2.5和S15组相比，S30组肝脏中生长激素受体-I的表达更高。肝脏中胰岛素样生长因子-I mRNA的表达随水体盐度的增加而增加，而在S15和S30组的肌肉中则下降。与S15组相比，S2.5组肝脏和肌肉中Δ6-去饱和酶的表达增加。与S15组相比，S30组肝脏中Δ6-去饱和酶的表达下降。与S2.5和S15组相比，S30处理使脑和肝脏中核结合蛋白2/nesfatin-1 mRNA的表达增加。观察到的基因表达变化可能有助于银汉鱼在盐度挑战期间表现得更好。长期来看，S30条件可能会促进银汉鱼的体细胞生长。
