Kozlov A P, Reshetnikov V L, Korzh V P, Neĭfakh A A
Mol Biol (Mosk). 1988 Nov-Dec;22(6):1614-22.
The fate of pAT153 DNA microinjected into the embryos of loach was examined. At the earliest stages of development (2 h) the high molecular weight transgenome consisting of pAT153 sequences is formed. The transgenome replicates intensively during the course of early development (until the blastula--early gastrula stages), but later the replication slows down which results in the elimination of the transgenome from the majority of embryos at more advanced stages. The analyses of the transgenome structure with the help of restriction endonucleases have shown that, after microinjection of linear DNA, the high molecular weight transgenome is formed by stochastic ligation of the sticky ends of injected molecules. Our data suggest that the transgenome formation from circular or supercoil DNA occurs after its linearization by host endonuclease.
对显微注射到泥鳅胚胎中的pAT153 DNA的命运进行了研究。在发育的最早阶段（2小时），由pAT153序列组成的高分子量转基因组形成。转基因组在早期发育过程中（直到囊胚期——原肠胚早期阶段）大量复制，但后来复制减缓，这导致在更晚期阶段大多数胚胎中转基因组被消除。借助限制性内切酶对转基因组结构的分析表明，线性DNA显微注射后，高分子量转基因组是由注射分子粘性末端的随机连接形成的。我们的数据表明，环状或超螺旋DNA的转基因组形成是在其被宿主内切酶线性化之后发生的。
