Beniumov A O, Enikolopov G N, Barmintsev V A, Zelenina I A, Sleptsova L A
Genetika. 1989 Jan;25(1):24-35.
Plasmid DNA containing human growth hormone gene was microinjected into cytoplasm of loach (Misgurnus fossilis L.) fertilized eggs. After plasmid injection, more than 50% of embryos reached the hatching stage. In control experiments embryogenesis was completed giving 72.5% of injected and 90% of intact larvae. Southern blot hybridization analysis revealed integration of injected recombinant DNA constructions into fish chromosome's DNA (2-30 copies per genome), without any significant rearrangements. Significant increase in length and weight of transgenic fish was observed in experiments (P less than 0.01). S1-analysis of RNA demonstrated correlation of the amount of specific RNA molecules and the accelerated growth of the individual specimens and proper utilization of the transcriptional start points in transgenic fish.
将含有人生长激素基因的质粒DNA显微注射到泥鳅（Misgurnus fossilis L.）受精卵的细胞质中。注射质粒后，超过50%的胚胎发育到孵化阶段。在对照实验中，胚胎发育完成，注射组幼虫孵化率为72.5%，未注射组为90%。Southern印迹杂交分析表明，注射的重组DNA构建体整合到鱼类染色体DNA中（每个基因组2 - 30个拷贝），且无明显重排。实验中观察到转基因鱼的体长和体重显著增加（P小于０.０１）。RNA的S1分析表明，转基因鱼中特定RNA分子的量与个体标本的加速生长以及转录起始点的正确利用相关。
