Ochiai Masaru, Misaki Kohei, Takeuchi Toshiki, Narumi Ryoyo, Azuma Yoshiyuki, Matsuo Tatsuhiro
School of Veterinary Medicine, Kitasato University.
Faculty of Agriculture, Kagawa University.
J Nutr Sci Vitaminol (Tokyo). 2017;63(2):111-119. doi: 10.3177/jnsv.63.111.
Egg white (EW) is known as a nutritional protein but can induce allergic reactions in humans. We investigated the dietary effects of EW and its hydrolysate (EWH), which contains less allergen, on body fat accumulation in Wistar rats fed an equicaloric high-fat and high-sucrose diet for 8 wk (Exp A). The pair-feeding of EW and equicaloric-feeding of EWH increased fecal fat excretion and suppressed lipid accumulation in the liver and muscles but not in the abdominal adipose tissues, carcass, or total body. Dietary EWH also suppressed the serum glucose level and alkaline phosphatase activity. Further, we showed a higher dispersibility of EW and EWH in physicochemical assay (Exp B). Next, we investigated the suppressive effects of a single administration of EW and EWH on lipid-induced hypertriglyceridemia and small intestinal meal transit in ddY mice (Exp C). However, a single administration of EW or EWH did not suppress the lipid-induced hypertriglyceridemia nor did it delay the rate of small intestinal transit. These findings indicated that dietary EW and EWH reduce hepatic and muscular (ectopic) fat accumulation mainly by suppressing fat absorption and supplying fat to the liver and muscles. Therefore, the low-allergenic EWH can be effective for the prevention of high-fat-diet-induced obesity.
蛋清（EW）是一种营养蛋白，但可在人体诱发过敏反应。我们研究了蛋清及其过敏原较少的水解产物（EWH），对喂饲等热量高脂肪高蔗糖饮食8周的Wistar大鼠体脂积累的饮食影响（实验A）。蛋清的配对喂养和EWH的等热量喂养增加了粪便脂肪排泄，并抑制了肝脏和肌肉中的脂质积累，但对腹部脂肪组织、胴体或全身没有影响。饮食中的EWH还抑制了血清葡萄糖水平和碱性磷酸酶活性。此外，我们在理化分析中显示了EW和EWH具有更高的分散性（实验B）。接下来，我们研究了单次给予EW和EWH对ddY小鼠脂质诱导的高甘油三酯血症和小肠食糜转运的抑制作用（实验C）。然而，单次给予EW或EWH既没有抑制脂质诱导的高甘油三酯血症，也没有延缓小肠转运速率。这些发现表明，饮食中的EW和EWH主要通过抑制脂肪吸收并将脂肪供应给肝脏和肌肉，来减少肝脏和肌肉（异位）脂肪积累。因此，低过敏原的EWH对预防高脂饮食诱导的肥胖可能有效。
