Hardung Stefanie, Alyahyay Mansour, Eriksson David, Diester Ilka
Ernst Strüngmann Institute gGmbHFrankfurt, Germany.
Faculty of Biology, Albert Ludwig University of FreiburgFreiburg, Germany.
Front Syst Neurosci. 2017 May 11;11:27. doi: 10.3389/fnsys.2017.00027. eCollection 2017.
Simultaneous recordings and manipulations of neural circuits that control the behavior of animals is one of the key techniques in modern neuroscience. Rapid advances in optogenetics have led to a variety of probes combining multichannel readout and optogenetic write in. Given the complexity of the brain, it comes as no surprise that the choice of the device is constrained by several factors such as the animal model, the structure and location of the brain area of interest, as well as the behavioral read out. Here we provide an overview of available devices for chronic simultaneous neural recordings and optogenetic manipulation in awake behaving rats. We focus on two fixed arrays and two moveable drives. For both options, we present data from one custom-made (in house) and one commercially available device. Here we provide evidence that simultaneous neural recordings and optogenetic manipulations are feasible with all four tested devices. Further we give detailed information about the recording quality, and also contrast the different features of the probes. As we provide detailed information about equipment and building procedures for combined chronic multichannel readout and optogenetic control with maximum performance at minimized costs, this overview might help especially researchers who want to enter the field of optophysiology.
同时记录和操纵控制动物行为的神经回路是现代神经科学的关键技术之一。光遗传学的迅速发展催生了多种结合多通道读出和光遗传学写入功能的探针。鉴于大脑的复杂性，设备的选择受到多种因素的限制就不足为奇了，这些因素包括动物模型、感兴趣脑区的结构和位置以及行为读出方式。在此，我们概述了可用于清醒行为大鼠慢性同时神经记录和光遗传学操纵的现有设备。我们重点介绍了两种固定阵列和两种可移动驱动器。对于这两种选择，我们展示了来自一种定制（内部自制）设备和一种商用设备的数据。在此我们提供证据表明，使用所有四种测试设备进行同时神经记录和光遗传学操纵都是可行的。此外，我们给出了关于记录质量的详细信息，并对比了探针的不同特性。由于我们提供了关于以最小成本实现最大性能的慢性多通道读出和光遗传学控制相结合的设备及构建程序的详细信息，本概述可能对尤其想要进入光生理学领域的研究人员有所帮助。
