Hong Daewha, Lee Hojae, Ko Eun Hyea, Lee Juno, Cho Hyeoncheol, Park Matthew, Yang Sung Ho, Choi Insung S
Center for Cell-Encapsulation Research , Department of Chemistry , KAIST , Daejeon 305-701 , Korea . Email:
Department of Chemistry Education , Korea National University of Education , Chungbuk 363-791 , Korea.
Chem Sci. 2015 Jan 1;6(1):203-208. doi: 10.1039/c4sc02789b. Epub 2014 Oct 1.
The cytoprotection of individual living cells under and daily-life conditions is a prerequisite for various cell-based applications including cell therapy, cell-based sensors, regenerative medicine, and even the food industry. In this work, we use a cytocompatible two-step process to encapsulate in a highly uniform nanometric (<100 nm) shell composed of organic poly(norepinephrine) and inorganic silica layers. The resulting cell-in-shell structure acquires multiple resistance against lytic enzyme, desiccation, and UV-C irradiation. In addition to the UV-C filtering effect of the double-layered shell, the biochemical responses of the encapsulated yeast are suggested to contribute to the observed UV-C tolerance. This work offers a chemical tool for cytoprotecting individual living cells under multiple stresses and also for studying biochemical behavior at the cellular level.
在日常生活条件下对单个活细胞进行细胞保护是包括细胞治疗、基于细胞的传感器、再生医学乃至食品工业在内的各种基于细胞的应用的前提条件。在这项工作中，我们采用一种细胞相容的两步法，将其封装在由有机聚（去甲肾上腺素）和无机二氧化硅层组成的高度均匀的纳米级（<100 nm）壳层中。所得的壳内细胞结构对裂解酶、干燥和UV-C辐射具有多重抗性。除了双层壳的UV-C过滤作用外，还表明封装酵母的生化反应有助于观察到的UV-C耐受性。这项工作提供了一种化学工具，用于在多种应激条件下对单个活细胞进行细胞保护，也用于研究细胞水平的生化行为。
