Department of Zoology, Faculty of Science, Assiut University, Assiut 71516, Egypt.
Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.
Ecotoxicol Environ Saf. 2017 Sep;143:344-350. doi: 10.1016/j.ecoenv.2017.05.013. Epub 2017 May 27.
Lead (Pb) is a toxic environmental pollutant that induces a broad range of biochemical and physiological hazards in living organisms. We investigated the possible hepatoprotective effects of Spirulina platensis (SP) in counteracting the Pb-induced oxidative damage. Ninety-six adult African catfish were allocated into four equal groups. The 1st group (control) fed basal diet while the 2nd group (Pb-treated) fed on basal diet and exposed to 1mg Pb(NO)/L. The 3rd and 4th groups fed SP-supplemented basal diets at levels of 0.25% and 0.5%, respectively and exposed to Pb. Serum samples were used to analyze hepatic function biomarkers, electrolytes, and oxidant and antioxidant status. Lipid peroxidation and DNA fragmentation were determined in the liver tissues. Pb exposure induced hepatic dysfunction, electrolytes (Na, K, Ca, and Cl) imbalance, as well a significant decrease in GSH content, and LDH, AChE, SOD, CAT and GST enzymes activity. SP supplementation reverted these biochemical and genetic alterations close to control levels. This amelioration was higher with 0.5% SP and at the 4th week of exposure, showing concentration- and time-dependency. Thus, the current study suggests that SP could protect the catfish liver against lead-induced injury by scavenging ROS, sustaining the antioxidant status and diminishing DNA oxidative damage. The dietary inclusion of SP can be used as a promising protective agent to counteract oxidative stress-mediated diseases and toxicities.
铅（Pb）是一种有毒的环境污染物，会对生物体造成广泛的生化和生理危害。我们研究了螺旋藻（SP）在对抗 Pb 诱导的氧化损伤方面的可能的保肝作用。96 条成年非洲鲶鱼被分配到四个相等的组中。第 1 组（对照组）喂食基础饮食，第 2 组（Pb 处理组）喂食基础饮食并暴露于 1mg Pb（NO₃）/L。第 3 组和第 4 组分别喂食含 0.25%和 0.5%SP 的基础饮食，并暴露于 Pb。血清样本用于分析肝功能生物标志物、电解质以及氧化和抗氧化状态。测定肝组织中的脂质过氧化和 DNA 片段化。Pb 暴露导致肝功能障碍、电解质（Na、K、Ca 和 Cl）失衡，以及 GSH 含量、LDH、AChE、SOD、CAT 和 GST 酶活性显著下降。SP 补充使这些生化和遗传变化恢复到接近对照水平。用 0.5%SP 进行补充的效果更高，在暴露的第 4 周，表现出浓度和时间依赖性。因此，本研究表明 SP 可以通过清除 ROS、维持抗氧化状态和减少 DNA 氧化损伤来保护鲶鱼肝免受 Pb 诱导的损伤。在饮食中添加 SP 可以作为一种有前途的保护剂，用于对抗氧化应激介导的疾病和毒性。
