Hamed Mohamed, Osman Alaa G M, Badrey Ahmed E A, Soliman Hamdy A M, Sayed Alaa El-Din H
Department of Zoology, Faculty of Science, Al Azhar University (Assiut Branch), Cairo, Egypt.
Department of Zoology, Faculty of Science, Sohag University, Sohag, Egypt.
Front Physiol. 2021 Sep 28;12:742922. doi: 10.3389/fphys.2021.742922. eCollection 2021.
This study aims to assess the impact of microplastics (MPs) on erythrocytes using eryptosis (apoptosis) and an erythron profile (poikilocytosis and nuclear abnormalities), considered to be novel biomarkers in Nile tilapia (. In this study, four groups of fish were used: The first was the control group. In the second group, 1 mg/L of MPs was introduced to the samples. The third group was exposed to 10 mg/L of MPs. Finally, the fourth group was exposed to 100 mg/L of MPs for 15 days, following 15 days of recovery. The fish treated with MPs experienced an immense rise in the eryptosis percentage, poikilocytosis, and nuclear abnormalities of red blood cells (RBCs) compared with the control group in a concentration-dependent manner. Poikilocytosis of MP-exposed groups included sickle cell shape, schistocyte, elliptocyte, acanthocyte, and other shapes. Nuclear abnormalities of the MPs-exposed groups included micronuclei, binucleated erythrocytes, notched, lobed, blebbed, and hemolyzed nuclei. After the recovery period, a greater percentage of eryptosis, poikilocytotic cells, and nuclear abnormalities in RBCs were still evident in the groups exposed to MPs when crosschecked with the control group. The results show concerning facts regarding the toxicity of MPs in tilapia.
本研究旨在利用红细胞凋亡（eryptosis）和红细胞谱系（异形红细胞症和核异常）来评估微塑料（MPs）对尼罗罗非鱼红细胞的影响，这些被认为是尼罗罗非鱼中新的生物标志物。在本研究中，使用了四组鱼：第一组为对照组。在第二组中，向样本中引入1 mg/L的微塑料。第三组暴露于10 mg/L的微塑料中。最后，第四组暴露于100 mg/L的微塑料中15天，之后恢复15天。与对照组相比，用微塑料处理的鱼红细胞凋亡百分比、异形红细胞症和红细胞（RBCs）核异常呈浓度依赖性大幅上升。暴露于微塑料组的异形红细胞症包括镰状细胞形状、裂红细胞、椭圆形红细胞、棘红细胞和其他形状。暴露于微塑料组的核异常包括微核、双核红细胞、有缺口的、分叶的、有泡的和溶血的细胞核。恢复期后，与对照组交叉核对时，暴露于微塑料组的红细胞凋亡、异形红细胞和核异常的比例仍更高。结果显示了有关微塑料对罗非鱼毒性的令人担忧的事实。
