School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
Department of Chemical Science and Engineering, Kobe University, Kobe 657-8501, Japan.
Bioresour Technol. 2017 Dec;245(Pt B):1447-1454. doi: 10.1016/j.biortech.2017.05.088. Epub 2017 May 18.
In this work, the consolidated bioprocessing (CBP) yeast Saccharomyces cerevisiae MNII/cocδBEC3 was transformed by an artificial zinc finger protein (AZFP) library to improve its thermal tolerance, and the strain MNII-AZFP with superior growth at 42°C was selected. Improved degradation of acid swollen cellulose by 45.9% led to an increase in ethanol production, when compared to the control strain. Moreover, the fermentation of Jerusalem artichoke stalk (JAS) by MNII-AZFP was shortened by 12h at 42°C with a concomitant improvement in ethanol production. Comparative transcriptomics analysis suggested that the AZFP in the mutant exerted beneficial effect by modulating the expression of multiple functional genes. These results provide a feasible strategy for efficient ethanol production from JAS and other cellulosic biomass through CBP based-fermentation at elevated temperatures.
在这项工作中，通过人工锌指蛋白（AZFP）文库对协同生物加工（CBP）酵母酿酒酵母 MNII/cocδBEC3 进行转化，以提高其耐热性，并选择了在 42°C 下生长更好的菌株 MNII-AZFP。与对照菌株相比，酸性膨胀纤维素的降解率提高了 45.9%，导致乙醇产量增加。此外，MNII-AZFP 发酵菊芋茎（JAS）时，在 42°C 下发酵时间缩短了 12h，同时乙醇产量也有所提高。比较转录组学分析表明，突变体中的 AZFP 通过调节多个功能基因的表达发挥有益作用。这些结果为通过高温下基于 CBP 的发酵从 JAS 和其他纤维素生物质中高效生产乙醇提供了一种可行的策略。
