Li Jun, Yu Ya-Fen, Liu Chang-Hua, Wang Cui-Mei
Department of Nephrology, The Affiliated Hospital of Jiangnan University, Wuxi 214062, Jiangsu, China.
Department of Nephrology, The Affiliated Hospital of Jiangnan University, Wuxi 214062, Jiangsu, China.
Pathol Res Pract. 2017 Sep;213(9):1215-1220. doi: 10.1016/j.prp.2017.04.011. Epub 2017 Apr 20.
CD163 and CD206, markers of M2 macrophages, possesses anti-inflammatory properties. This study aims to investigate the clinicopathologic significance of M2 macrophages in patients of glomerulonephritis with crescents.
Renal tissue samples from patients of glomerulonephritis with more than 30% cell or cell-fibrous crescents, including lupus nephritis (LN, n=14), anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV, n=14), IgA nephropathy(IgAN) (n=11), Henoch Schonlein purpura glomerulonephritis(HSPGN)(n=8)were included in this study. The expression of CD163, CD206 and CD68 in renal tissues was detected by immunohistochemistry or immunofluorescence.
(1) CD163 was mainly expressed in cell or cell-fibrous crescents, proliferative glomerular lesions and acute tubulointerstitial injury. There were numerous CD163-positive cells in LN and AAV in comparison with IgAN and HSPGN. (2) CD206-positive cells were mainly observed in acute tubulointerstitial injury, and proliferative glomerular lesions, especially in LN. Patients with LN had numerous CD206-positive cells in glomerular than other groups. The number of CD163-positive cells and CD206-positive cells in acute tubulointerstitial lesions of LN and AAV were more than IgAN and HSPGN. (3) Both the number of CD163-positive cells and CD206-positive cells in acute tubulointerstitial lesions negatively correlated to estimated glomerular filtration rate. (4) In LN, activity index (AI) positively correlated with the number of CD206-positive cells and CD163-positive cells. Dual staining showed that CD163-positive cells and CD206-positive cells also expressed CD68.
CD163-positive cells and CD206-positive cells, subpopulation of macrophages, which were involved in the pathogenesis of active crescentic glomerulonephritis, especially in LN and AAV.
M2巨噬细胞标志物CD163和CD206具有抗炎特性。本研究旨在探讨M2巨噬细胞在伴有新月体的肾小球肾炎患者中的临床病理意义。
纳入细胞或细胞纤维性新月体超过30%的肾小球肾炎患者的肾组织样本，包括狼疮性肾炎（LN，n = 14）、抗中性粒细胞胞浆抗体相关性血管炎（AAV，n = 14）、IgA肾病（IgAN）（n = 11）、过敏性紫癜性肾小球肾炎（HSPGN）（n = 8）。通过免疫组织化学或免疫荧光检测肾组织中CD163、CD206和CD68的表达。
（1）CD163主要表达于细胞或细胞纤维性新月体、增殖性肾小球病变及急性肾小管间质损伤部位。与IgAN和HSPGN相比，LN和AAV中有大量CD163阳性细胞。（2）CD206阳性细胞主要见于急性肾小管间质损伤及增殖性肾小球病变，尤其是LN。LN患者肾小球中CD206阳性细胞比其他组多。LN和AAV急性肾小管间质病变中CD163阳性细胞和CD206阳性细胞的数量多于IgAN和HSPGN。（3）急性肾小管间质病变中CD163阳性细胞和CD206阳性细胞的数量均与估计肾小球滤过率呈负相关。（4）在LN中，活动指数（AI）与CD206阳性细胞和CD163阳性细胞的数量呈正相关。双重染色显示CD163阳性细胞和CD206阳性细胞也表达CD68。
CD163阳性细胞和CD206阳性细胞作为巨噬细胞亚群，参与了活动性新月体性肾小球肾炎的发病机制，尤其是在LN和AAV中。
