Diwan Vishal, Purohit Manju, Chandran Salesh, Parashar Vivek, Shah Harshada, Mahadik Vijay K, Stålsby Lundborg Cecilia, Tamhankar Ashok J
Department of Public Health and Environment, R.D. Gardi Medical College, Ujjain 456006, India.
Department of Public Health Sciences, Global Health, Health Systems and Policy (HSP): Medicines focusing on antibiotics, Karolinska Institutet, Stockholm 17177, Sweden.
Int J Environ Res Public Health. 2017 May 29;14(6):574. doi: 10.3390/ijerph14060574.
Antibiotic resistance (ABR) is one of the major health emergencies for global society. Little is known about the ABR of environmental bacteria and therefore it is important to understand ABR reservoirs in the environment and their potential impact on health.
METHOD/DESIGN: Quantitative and qualitative data will be collected during a 3-year follow-up study of a river associated with religious mass-bathing in Central India. Surface-water and sediment samples will be collected from seven locations at regular intervals for 3 years during religious mass-bathing and in absence of it to monitor water-quality, antibiotic residues, resistant bacteria, antibiotic resistance genes and metals. Approval has been obtained from the Ethics Committee of R.D. Gardi Medical College, Ujjain, India (No. 2013/07/17-311).
The results will address the issue of antibiotic residues and antibiotic resistance with a focus on a river environment in India within a typical socio-behavioural context of religious mass-bathing. It will enhance our understanding about the relationship between antibiotic residue levels, water-quality, heavy metals and antibiotic resistance patterns in Escherichia coli isolated from river-water and sediment, and seasonal differences that are associated with religious mass-bathing. We will also document, identify and clarify the genetic differences/similarities relating to phenotypic antibiotic resistance in bacteria in rivers during religious mass-bathing or during periods when there is no mass-bathing.
抗生素耐药性是全球社会面临的主要健康紧急情况之一。人们对环境细菌的抗生素耐药性知之甚少，因此了解环境中的抗生素耐药性储存库及其对健康的潜在影响非常重要。
方法/设计：在对印度中部与宗教集体沐浴相关的一条河流进行的为期3年的随访研究中，将收集定量和定性数据。在宗教集体沐浴期间和非宗教集体沐浴期间，将每隔一段时间从七个地点采集地表水和沉积物样本，为期3年，以监测水质、抗生素残留、耐药细菌、抗生素耐药基因和金属。已获得印度乌贾因R.D.加尔迪医学院伦理委员会的批准（编号2013/07/17 - 311）。
研究结果将聚焦于印度河流环境以及宗教集体沐浴这一典型社会行为背景下的抗生素残留和抗生素耐药性问题。这将增进我们对从河水和沉积物中分离出的大肠杆菌中抗生素残留水平、水质、重金属与抗生素耐药模式之间关系的理解，以及与宗教集体沐浴相关的季节差异。我们还将记录、识别并阐明在宗教集体沐浴期间或无集体沐浴期间河流中细菌表型抗生素耐药性相关的遗传差异/相似性。
