Chang Hao-Xun, Hartman Glen L
Department of Plant, Soil, and Microbial Sciences, Michigan State UniversityEast Lansing, MI, USA.
United States Department of Agriculture - Agricultural Research Service, University of IllinoisUrbana, IL, USA.
Front Plant Sci. 2017 May 15;8:670. doi: 10.3389/fpls.2017.00670. eCollection 2017.
Management of insects that cause economic damage to yields of soybean mainly rely on insecticide applications. Sources of resistance in soybean plant introductions (PIs) to different insect pests have been reported, and some of these sources, like for the soybean aphid (SBA), have been used to develop resistant soybean cultivars. With the availability of SoySNP50K and the statistical power of genome-wide association studies, we integrated phenotypic data for beet armyworm, Mexican bean beetle (MBB), potato leafhopper (PLH), SBA, soybean looper (SBL), velvetbean caterpillar (VBC), and chewing damage caused by unspecified insects for a comprehensive understanding of insect resistance in the United States Department of Agriculture Soybean Germplasm Collection. We identified significant single nucleotide (SNP) polymorphic markers for MBB, PLH, SBL, and VBC, and we highlighted several leucine-rich repeat-containing genes and myeloblastosis transcription factors within the high linkage disequilibrium region surrounding significant SNP markers. Specifically for soybean resistance to PLH, we found the PLH locus is close but distinct to a locus for soybean pubescence density on chromosome 12. The results provide genetic support that pubescence density may not directly link to PLH resistance. This study offers a novel insight of soybean resistance to four insect pests and reviews resistance mapping studies for major soybean insects.
对大豆产量造成经济损失的昆虫的管理主要依赖于杀虫剂的使用。已有报道称大豆植物引进品种（PI）对不同害虫具有抗性来源，其中一些来源，如对大豆蚜（SBA）的抗性来源，已被用于培育抗性大豆品种。随着SoySNP50K的出现以及全基因组关联研究的统计能力，我们整合了甜菜夜蛾、墨西哥豆瓢虫（MBB）、马铃薯叶蝉（PLH）、SBA、大豆尺蠖（SBL）、豆蚀叶野螟（VBC）的表型数据以及未明确昆虫造成的咀嚼损伤数据，以便全面了解美国农业部大豆种质库中的昆虫抗性。我们鉴定出了与MBB、PLH、SBL和VBC相关的显著单核苷酸（SNP）多态性标记，并突出了显著SNP标记周围高连锁不平衡区域内的几个富含亮氨酸重复序列的基因和成髓细胞瘤转录因子。具体而言，对于大豆对PLH的抗性，我们发现PLH基因座与第12号染色体上大豆茸毛密度基因座相近但不同。结果提供了遗传证据，表明茸毛密度可能与PLH抗性没有直接联系。本研究为大豆对四种害虫的抗性提供了新的见解，并综述了主要大豆害虫的抗性图谱研究。
