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比较转录组学揭示了与八倍体草莓抗性相关的基因差异表达。

Comparative Transcriptomics Reveals Differential Gene Expression Related to Resistance in the Octoploid Strawberry.

作者信息

Wang Feng, Zhang Feng, Chen Mengmeng, Liu Zhiheng, Zhang Zhihong, Fu Junfan, Ma Yue

机构信息

College of Plant Protection, Shenyang Agricultural UniversityShenyang, China.

College of Horticulture, Shenyang Agricultural UniversityShenyang, China.

出版信息

Front Plant Sci. 2017 May 15;8:779. doi: 10.3389/fpls.2017.00779. eCollection 2017.

Abstract

The strawberry is an important fruit worldwide; however, the development of the strawberry industry is limited by fungal disease. Anthracnose is caused by the pathogen and leads to large-scale losses in strawberry quality and production. However, the transcriptional response of strawberry to infection with is poorly understood. In the present study, the strawberry leaf transcriptome of the 'Yanli' and 'Benihoppe' cultivars were deep sequenced via an RNA-seq analysis to study resistance in strawberry. Among the sequences, differentially expressed genes were annotated with Gene Ontology terms and subjected to pathway enrichment analysis. Significant categories included defense, plant-pathogen interactions and flavonoid biosynthesis were identified. The comprehensive transcriptome data set provides molecular insight into resistance genes in resistant and susceptible strawberry cultivars. Our findings can enhance breeding efforts in strawberry.

摘要

草莓是全球重要的水果;然而,草莓产业的发展受到真菌病害的限制。炭疽病由病原体引起,会导致草莓品质和产量大幅损失。然而,草莓对该病原体感染的转录反应尚不清楚。在本研究中,通过RNA测序分析对‘艳丽’和‘红颜’品种的草莓叶片转录组进行了深度测序,以研究草莓的抗性。在这些序列中,差异表达基因用基因本体术语进行注释,并进行通路富集分析。鉴定出了包括防御、植物-病原体相互作用和类黄酮生物合成在内的重要类别。该综合转录组数据集为抗性和易感草莓品种中的抗性基因提供了分子层面的见解。我们的研究结果可以加强草莓的育种工作。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5ac/5430163/665c81138e0c/fpls-08-00779-g001.jpg

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