Lu Danying, Liu Bin, Ren Mingjie, Wu Chao, Ma Jingjing, Shen Yamei
Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou 311300, China.
College of Landscape and Architecture, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Plants (Basel). 2021 Oct 22;10(11):2261. doi: 10.3390/plants10112261.
The endangered plant largely grows in the understory of forest and suffers light deficiency stress. It is generally recognized that the interaction between plant development and growth environment is intricate; however, the underlying molecular regulatory pathways by which light deficiency induced growth inhibition remain obscure. To understand the physiological and molecular mechanisms of plant response to shading caused light deficiency, we performed photosynthesis efficiency analysis and comparative transcriptome analysis in leaves, which were subjected to shading treatments of different durations. Most of the parameters relevant to the photosynthesis systems were altered as the result of light deficiency treatment, which was also confirmed by the transcriptome analysis. Gene Ontology and KEGG pathway enrichment analyses illustrated that most of differential expression genes (DEGs) were enriched in photosynthesis-related pathways. Light deficiency may have accelerated leaf abscission by impacting the photosynthesis efficiency and hormone signaling. Further, shading could repress the expression of stress responsive transcription factors and R-genes, which confer disease resistance. This study provides valuable insight into light deficiency-induced molecular regulatory pathways in and offers a theoretical basis for conservation and cultivation improvements of and other endangered woody plants.
这种濒危植物主要生长在森林下层，遭受光照不足胁迫。人们普遍认为植物发育与生长环境之间的相互作用错综复杂；然而，光照不足诱导生长抑制的潜在分子调控途径仍不清楚。为了解植物对遮荫导致的光照不足的生理和分子机制，我们对经过不同时长遮荫处理的叶片进行了光合作用效率分析和比较转录组分析。光照不足处理导致大多数与光合作用系统相关的参数发生变化，转录组分析也证实了这一点。基因本体论和KEGG通路富集分析表明，大多数差异表达基因（DEG）富集在与光合作用相关的通路中。光照不足可能通过影响光合作用效率和激素信号传导加速叶片脱落。此外，遮荫会抑制赋予抗病性的应激反应转录因子和R基因的表达。本研究为光照不足诱导的分子调控途径提供了有价值的见解，并为该植物以及其他濒危木本植物的保护和栽培改良提供了理论依据。
