Ren Tianheng, Tang Zongxiang, Fu Shulan, Yan Benju, Tan Feiquan, Ren Zhenglong, Li Zhi
Agronomy College, Sichuan Agricultural UniversitySichuan, China.
College of Life Science, Sichuan Agricultural UniversitySichuan, China.
Front Plant Sci. 2017 May 15;8:799. doi: 10.3389/fpls.2017.00799. eCollection 2017.
Rye has been used worldwide as a source for the genetic improvement of wheat. In this study, two stable wheat-rye primary T1RS.1BL translocation lines were selected from the progeny of the crossing of the wheat cultivar Mianyang11-1 and a Chinese local rye variety, Weining. These two novel translocation lines were identified by molecular cytogenetic analysis. PCR results, multi-color fluorescence hybridization (MC-FISH), and acid polyacrylamide gel electrophoresis (A-PAGE) indicated that both new translocation lines harbor a pair of T1RS.1BL translocation chromosomes, and have been named RT828-10 and RT828-11, respectively. The cytogenetic results also indicated that the pSc119.2 signals of 5AL were absent in both lines along with the pSc119.2 signals of 4AL of RT828-11. When inoculated with different stripe rust and powdery mildew isolates, both lines expressed high resistance to f. sp. and f. sp. pathotypes, which are prevalent in China and are virulent on . The line RT828-11 also exhibited excellent agronomic traits in the field. The present study indicates that this rye variety may carry untapped variations that could potentially be used for wheat improvement.
黑麦已在全球范围内被用作小麦遗传改良的资源。在本研究中，从小麦品种绵阳11-1与中国地方黑麦品种威宁杂交后代中筛选出两个稳定的小麦-黑麦初级T1RS.1BL易位系。通过分子细胞遗传学分析鉴定了这两个新型易位系。PCR结果、多色荧光原位杂交（MC-FISH）和酸性聚丙烯酰胺凝胶电泳（A-PAGE）表明，这两个新的易位系均含有一对T1RS.1BL易位染色体，分别命名为RT828-10和RT828-11。细胞遗传学结果还表明，两个系中5AL的pSc119.2信号均缺失，RT828-11中4AL的pSc119.2信号也缺失。当接种不同的条锈菌和白粉菌分离株时，两个系对在中国普遍存在且对……具有毒性的……和……致病型均表现出高抗性。RT828-11系在田间也表现出优异的农艺性状。本研究表明，该黑麦品种可能携带未开发的变异，可潜在地用于小麦改良。
